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The RNA Polymerase a Subunit from Sinorhizobium meliloti Can Assemble with RNA Polymerase Subunits from Escherichia coli and Function in Basal and Activated Transcription both In Vivo and In Vitro

Peck, M.C.; Gaal, T.; Fisher, R.F.

Journal of Bacteriology 184(14): 08-14

2002


ISSN/ISBN: 0021-9193
Accession: 009998963

The gene for the a subunit of RNA polymerase was cloned and characterized to facilitate studies of transcription in the gram- negative soil bacterium Sinorhizobium meliloti. rpoA coded for a 336-amino-acid 37 kDa protein, and the region surrounding the gene contained 6 open reading frames in the conserved order secY (SecY)-adk (Adk)-rpsM (S13)- rpsK (S11)-rpoA (a)-rplQ (L17). S. meliloti a functions were conserved in heterologous host Escherichia coli even though the 2 a subunits were only 51 percent identical. The use of E. coli as a heterologous system in which to study the regulation of S. meliloti genes could represent an important tool for understanding and manipulating these processes.

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