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The RNA Polymerase a Subunit from Sinorhizobium meliloti Can Assemble with RNA Polymerase Subunits from Escherichia coli and Function in Basal and Activated Transcription both In Vivo and In Vitro

Peck, M.C.; Gaal, T.; Fisher, R.F.

Journal of Bacteriology 184(14): 08-14

2002

ISSN/ISBN: 0021-9193
Accession: 009998963
The gene for the a subunit of RNA polymerase was cloned and characterized to facilitate studies of transcription in the gram- negative soil bacterium Sinorhizobium meliloti. rpoA coded for a 336-amino-acid 37 kDa protein, and the region surrounding the gene contained 6 open reading frames in the conserved order secY (SecY)-adk (Adk)-rpsM (S13)- rpsK (S11)-rpoA (a)-rplQ (L17). S. meliloti a functions were conserved in heterologous host Escherichia coli even though the 2 a subunits were only 51 percent identical. The use of E. coli as a heterologous system in which to study the regulation of S. meliloti genes could represent an important tool for understanding and manipulating these processes.

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Related References

Peck, M.C.; Gaal, T.; Fisher, R.F.; Gourse, R.L.; Long, S.R. 2002: The RNA polymerase alpha subunit from Sinorhizobium meliloti can assemble with RNA polymerase subunits from Escherichia coli and function in basal and activated transcription both in vivo and in vitro Journal of Bacteriology 184(14): 3808-3814
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