+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

The positive activator of cephamycin C and clavulanic acid production in Streptomyces clavuligerus is mistranslated in a bldA mutant



The positive activator of cephamycin C and clavulanic acid production in Streptomyces clavuligerus is mistranslated in a bldA mutant



Microbiology 148(Pt 3): 643-656



In Streptomyces coelicolor bldA encodes the principal leucyl tRNA for translation of UUA codons and controls pigmented antibiotic production by the presence of TTA codons in the genes encoding the pathway-specific activators of actinorhodin and undecylprodigiosin biosynthesis. In Streptomyces clavuligerus the gene encoding the pathway-specific activator of both cephamycin C and clavulanic acid production, ccaR, also contains a TTA codon and was expected to exhibit bldA-dependence. A cloned S. clavuligerus DNA fragment containing a sequence showing 91% identity to the S. coelicolor bldA-encoded tRNA was able to restore antibiotic production and sporulation to bldA mutants of S. coelicolor and the closely related Streptomyces lividans. A null mutation of the bldA gene in S. clavuligerus resulted in the expected sporulation defective phenotype, but unexpectedly had no effect on antibiotic production. Transcript analysis showed no difference in the levels of ccaR transcripts in the wild-type and bldA mutant strains, ruling out any effect of elevated levels of the ccaR mRNA. Furthermore, when compared to the wild-type strain, the bldA mutant showed no differences in the levels of CcaR, suggesting that the single TTA codon in ccaR is mistranslated efficiently. The role of codon context in bldA dependence is discussed. Reprinted by permission of the publisher.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 010010240

Download citation: RISBibTeXText

PMID: 11882698


Related references

Expression of ccaR, encoding the positive activator of cephamycin C and clavulanic acid production in Streptomyces clavuligerus, is dependent on bldG. Antimicrobial Agents and ChemoTherapy 49(4): 1529-1541, 2005

Coordinate production of cephamycin c and clavulanic acid by Streptomyces clavuligerus. Indian Journal of Experimental Biology 37(10): 1031-1033, 1999

Production of clavulanic acid and cephamycin C by Streptomyces clavuligerus in palm-oil medium. World Journal of Microbiology and Biotechnology 12(1): 73-75, 1996

Phosphate repression of cephamycin and clavulanic acid production by streptomyces clavuligerus. Applied Microbiology & Biotechnology 26(2): 130-135, 1987

A regulatory gene (ccaR) required for cephamycin and clavulanic acid production in Streptomyces clavuligerus: amplification results in overproduction of both b-lactam compounds. Journal of Bacteriology 179(6): 53-9, 1997

A regulatory gene (ccaR) required for cephamycin and clavulanic acid production in Streptomyces clavuligerus: amplification results in overproduction of both beta-lactam compounds. Journal of Bacteriology 179(6): 2053-2059, 1997

Dissociation of cephamycin and clavulanic acid biosynthesis in streptomyces clavuligerus. Applied Microbiology & Biotechnology 20(5): 318-325, 1984

Dissociation of cephamycin C and clavulanic acid biosynthesis by 1,3-diaminopropane in Streptomyces clavuligerus. Fems Microbiology Letters 363(1): Fnv215, 2016

Improvement for the production of clavulanic acid by mutant Streptomyces clavuligerus. Letters in Applied Microbiology 34(5): 370-375, 2002

Characterization of DNA-binding sequences for CcaR in the cephamycin-clavulanic acid supercluster of Streptomyces clavuligerus. Molecular Microbiology 81(4): 968-981, 2011

Isolation and biochemical characterization of streptomyces clavuligerus mutants in the biosynthesis of clavulanic acid and cephamycin c. Applied Microbiology & Biotechnology 27(5-6): 510-516, 1988

Fed-batch cultivation for the production of clavulanic acid by an immobilized Streptomyces clavuligerus mutant. World Journal of Microbiology & Biotechnology 17(9): 869-872, 2001

Clavulanic acid production by the MMS 150 mutant obtained from wild type Streptomyces clavuligerus ATCC 27064. Brazilian Journal of Microbiology 44(4): 1049-1057, 2013

Streptomyces clavuligerus relA-null mutants overproduce clavulanic acid and cephamycin C: negative regulation of secondary metabolism by (p)ppGpp. Microbiology 154(Pt 3): 744-755, 2008