Transcription activation by a variety of AraC/XylS family activators does not depend on the class II-specific activation determinant in the N-terminal domain of the RNA polymerase alpha subunit
Egan, S.M.; Pease, A.J.; Lang, J.; Li, X.; Rao, V.; Gillette, W.K.; Ruiz, R.; Ramos, J.L.; Wolf, R.E.
Journal of Bacteriology 182(24): 7075-7077
ISSN/ISBN: 0021-9193 PMID: 11092872 DOI: 10.1128/jb.182.24.7075-7077.2000
The N-terminal domain of the RNA polymerase asubunit (a-NTD) was tested for a role in transcription activation by a variety of AraC/XylS family members. Based on substitutions at residues 162 to 165 and an extensive genetic screen we conclude that a-NTD is not an activation target for these activators. Reprinted by permission of the publisher.