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Trypsin mediates growth phase-dependent transcriptional regulation of genes involved in biosynthesis of ruminococcin A, a lantibiotic produced by Ruminococcus gnavus strain from a human intestinal microbiota


Trypsin mediates growth phase-dependent transcriptional regulation of genes involved in biosynthesis of ruminococcin A, a lantibiotic produced by Ruminococcus gnavus strain from a human intestinal microbiota



Journal of Bacteriology 184(1): 28



ISSN/ISBN: 0021-9193

The genetic requirements for ruminococcin A (RumA) biosynthesis and the role of trypsin in RumA production by the intestinal bacterium Ruminococcus gnavus E1 were investigated. RumA is a trypsin-dependent lantibiotic that has activity against enteropathogenic clostridia. A 12.8 kb DNA fragment containing the RumA locus was cloned and sequenced, and its transcriptional organization was determined under inducing and noninducing conditions. Trypsin activity was necessary for the growth phase-dependent transcriptional activation of RumA operons. The findings indicate that bacteriocin production by R. gnavus E1 is controlled via a complex signaling mechanism involving the proteolytic processing of a putative extracellular inducer-peptide by trypsin, a specific environmental cue of the digestive ecosystem.

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Accession: 010021675

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Related references

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