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Urogenital mycoplasmal infections: Comparative evaluation of test kits Mycoplasma IST, Mycoplasma-Kit and ATB mycoplasmas used in isolation, identification, titration and antibiogram



Urogenital mycoplasmal infections: Comparative evaluation of test kits Mycoplasma IST, Mycoplasma-Kit and ATB mycoplasmas used in isolation, identification, titration and antibiogram



Feuillets de Biologie 35(201): 35-40



The value of Mycoplasma IST (bioMerieux) test kit in the isolation, identification, titration and antibiogram of urogenital mycoplasma infections was assessed. one hundred twenty-eight samples of vaginal, sperm, Douglas fluids and placenta were tested and compared with the classical method (urea-arginine culture medium, A7 agar and ATB mycoplasmas). Good concordance of both techniques was observed in identification and quantitative analysis of mycoplasmas. One strain, Mycoplasma hominis, could not be detected by the new method, as growth did not occur in liquid medium. Good results were observed in the assessment of antibiotic sensitivity using the ATB mycoplasmas kit. Certain discrepancy between the two methods was recorded for erythromycin, mostly due to different critical concentrations. In general, this new method was found to be easy to use, reliable and featured a good conservation of lyophilized reagents.

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