Yield of haploid plants of spring wheat (Triticum aestivum L.) in anther culture in vitro depending on composition of sugars in nutrient medium

Molkanova, O.I.; Danilova, T.V.

Izvestiya Timiryazevskoi Sel'skokhozyaistvennoi Akademii 1994(4): 69-75


Accession: 010033470

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Practical application of androgenetic haploids is still limited by a small number of haploid individuals recovered from others. We compared 6 mediums (with sucrose, maltose and glucose, liquid and solid ones and 5 genotypes of spring wheat (F-1 hybrids). In the following experiment we studied the dynamics of hydrolysis and utilization of carbohydrates in 3 liquid mediums in a more responsive genotype. The liquid glucose medium was best. Using this medium we obtained greed haploid plants from breeding material which was initially nonresponsive. Liquid mediums contained 3 different carbohydrates, they differed in their osmotic regimes and in composition of utilized carbohydrates.