A carboxy-terminal 16-amino-acid region of sigma (38) of Escherichia coli is important for transcription under high-salt conditions and sigma activities in vivo

Ohnuma, M.; Fujita, N.; Ishihama, A.; Tanaka, K.; Takahashi, H.

Journal of Bacteriology 182(16): 4628-4631

2000


ISSN/ISBN: 0021-9193
PMID: 10913098
DOI: 10.1128/jb.182.16.4628-4631.2000
Accession: 010048530

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Abstract
sigma(38) (or sigma(S), the rpoS gene product) is a sigma subunit of RNA polymerase in Escherichia coli and directs transcription from a number of stationary-phase promoters as well as osmotically inducible promoters. In this study, we analyzed the function of the carboxy-terminal 16-amino-acid region of sigma(38) (residues 315 to 330), which is well conserved among the rpoS gene products of enteric bacterial species. Truncation of this region was shown to result in the loss of sigma activity in vivo using promoter-lacZ fusion constructs, but the mutant sigma(38) retained the binding activity in vivo to the core enzyme. The in vitro transcription analysis revealed that the transcription activity of sigma(38) holoenzyme under high potassium glutamate concentrations was significantly decreased by the truncation of the carboxy-terminal tail element.