A rapid technique for analysis of formalin-fixed, paraffin-embedded tissues by fluorescent in situ hybridization with alpha-satellite probes

Barril, N.; Carvalho Salles, A.B.; Tajara, E.H.

Genetics and Molecular Biology 21(4): 447-448

1998


ISSN/ISBN: 1415-4757
Accession: 010092216

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Abstract
We describe a rapid procedure for preparing archival tissues for interphase FISH analysis. The present protocol differs from others previously described because it allows the obtention of nuclei satisfactory number and quality without using special equipments, adhesive-treated slides or solutions for chromatin decondensation. The method is of low cost and useful for retrospective analyses of formalin-fixed, paraffin-embedded samples.