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Adenosine triphosphate mobilizes cytosolic calcium and modulates ionic currents in mouse taste receptor cells

Adenosine triphosphate mobilizes cytosolic calcium and modulates ionic currents in mouse taste receptor cells

Neuroscience Letters 290(3): 165-168

By using photometry and the patch clamp technique, we identified P2Y-like receptors in mouse taste receptor cells (TRCs) and found them to be coupled to Ca2+ mobilization and ionic current modulation. Particularly, adenosine triphosphate (ATP) and the P2Y agonist 2-methylthio-ATP increased intracellular Ca2+ by stimulating the phosphoinositide pathway, whereas beta,gamma-methylene-D-ATP, a P2X agonist, was ineffective. In a distinctive TRC subpopulation, ATP closed Ca2+ channels. This regulation may underlie the negative feedback tuning neurotransmitter release. By mobilizing intracellular Ca2+, ATP activated Ca2+-dependent Cl- channels, the intracellular event that may universally occur upon taste stimulation triggering IP3 formation and Ca2+ release in the TRC cytoplasm.

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Accession: 010130269

Download citation: RISBibTeXText

PMID: 10963889

DOI: 10.1016/s0304-3940(00)01342-2

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