+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Alterations of wheat root plasma membrane lipid composition induced by copper stress result in changed physicochemical properties of plasma membrane lipid vesicles



Alterations of wheat root plasma membrane lipid composition induced by copper stress result in changed physicochemical properties of plasma membrane lipid vesicles



Biochimica et biophysica acta = International journal of biochemistry and biophysics, 1564(2): 466-472



A response when wheat is grown in excess copper is an altered lipid composition of the root plasma membrane (PM). With detailed characterisation of the root PM lipid composition of the copper-treated plants as a basis, in the present study, model systems were used to gain a wider understanding about membrane behaviour, and the impact of a changed lipid composition. PMs from root cells of plants grown in excess copper (50 micromolar Cu(2+)) and control (0.3 micromolar Cu(2+)) were isolated using the two-phase partitioning method. Membrane vesicles were prepared of total lipids extracts from the isolated PMs, and also reference vesicles of phosphatidylcholine (PC). In a series of tests, the vesicle permeability for glucose and for protons was analysed. The vesicles show that copper stress reduced the permeability for glucose of the lipid bilayer barrier. When vesicles from stressed plants were modified by addition of lipids to resemble vesicles from control plants, the permeability for glucose was very similar to that of vesicles from control plants. The permeability for protons did not change upon stress. Electron paramagnetic resonance (EPR) of the lipid vesicles spin probed with n-doxylstearic acid (nDSA) was used to explore the lipid rotational freedom at different depth of the bilayer. The EPR measurements supported the permeability data, indicating that the copper stress resulted in more tightly packed bilayers of the PMs with reduced acyl chain motion.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 010147857

Download citation: RISBibTeXText

PMID: 12175930

DOI: 10.1016/s0005-2736(02)00498-4


Related references

The C-terminal CGHC motif of protein disulfide isomerase supports thrombosis. Journal of Clinical Investigation 125(12): 4391-4406, 2015

Changes in plasma, erythrocyte, and platelet magnesium levels in normotensive and hypertensive obese subjects during oral glucose tolerance test. American Journal of Hypertension 12(2 Pt 1): 128-136, 1999

Regulation and adaptation of the photosynthetic system. Sveriges Utsadesforenings Tidskrift 94(2): 95-102, 1984

Plasma membrane intrinsic proteins of Beta vulgaris L. Plant Physiology 108(1): 387-392, 1995

Effects of lithium salts on hyper thyroidism with mental symptoms. Folia Psychiatrica et Neurologica Japonica 37(3): 401, 1983

The relationship between plasma membrane lipid composition and physical-chemical properties. I. Fluorescence polarization studies of fatty acid-altered EL4 tumor cell membranes. Biochimica et Biophysica Acta 642(1): 106-118, 1981

Effects of calcium infiltration on quality, physical-biochemistry and ultrastructure of Starkrimson apples. Acta Agriculturae Boreali Sinica 13(Suppl.): 76-80, 1998

Effects of saline sprays on symptoms after endoscopic sinus surgery. American Journal of Rhinology 20(2): 191-196, 2006

Differential transcript abundance of salt overly sensitive (SOS) pathway genes is a determinant of salinity stress tolerance of wheat. Acta Physiologiae Plantarum 37(8): 169, 2015

Lipid composition of 30 species of yeast. Lipids 11(12): 837-844, 1976

Altered lipid parameters in patients infected with Entamoeba histolytica, Entamoeba dispar and Giardia lamblia. British Journal of Biomedical Science 62(2): 63-65, 2005

Effects of exogenous equine somatotropin on mineral balance in two-year-old horses in race training. Journal of Equine Veterinary Science 20(3): 201-206, 2000

Stress tolerance in some Chacoan dolichoderine ants: Implications for community organization and distribution. Journal of Arid Environments 35(2): 297-310, 1997

Xenobiotic-induced aberrations of lipid metabolism. Fatty acids in foods and their health implications edited by Ching Kuang Chow: 630, 1992

Xenobiotic contaminants and the deleterious effects of fats. Biochemical Society Transactions 17(3): 475-476, 1989