Aspirin dose dependently inhibits the interleukin-1 beta-stimulated increase in inducible nitric oxide synthase, nitric oxide, and prostaglandin E (2) production in rat ovarian dispersates cultured in vitro

Carnovale, D.E.; Fukuda, A.; Underhill, D.C.; Laffan, J.J.; Breuel, K.F.

Fertility and Sterility 75(4): 778-784

2001


ISSN/ISBN: 0015-0282
PMID: 11287034
DOI: 10.1016/s0015-0282(00)01784-2
Accession: 010205152

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Abstract
Objective: Determine if aspirin inhibits the IL-1beta-stimulated expression of inducible nitric oxide synthase (iNOS), nitric oxide (NO), and prostaglandin E2 (PGE2) in rat ovarian dispersates cultured in vitro. Design: Prospective, controlled in vitro study. Setting: Academic research laboratory. Animals: Ovaries collected from immature rats. Intervention(s): Ovaries were collected from immature rats and enzymatically dispersed. Ovarian dispersates were placed into plates containing media alone or media supplemented with IL-1beta (100 U/mL) and varying concentrations of aspirin (0, 1, 3, 5 and 10 mM). Ovarian dispersates were cultured in a humidified environment of 5% CO2 in air at 37degreeC for 24 or 48 hours. Main Outcome Measure(s): Twenty-four- and 48-hour iNOS, nitrite (a stable metabolite of NO), and PGE2 levels were determined from ovarian dispersates cultured in vitro. Result(s): Administration of IL-1beta increased nitrite and PGE2 levels over that observed in the control group after culture of ovarian dispersates for 24 and 48 hours. Aspirin dose dependently reduced the IL-1beta-stimulated increase in nitrite production from ovarian dispersates after culture for 24 and 48 hours. Aspirin completely (24 hours) or dose dependently (48 hours) prevented the IL-beta-stimulated increase in PGE2. Coadministration of IL-1beta and aspirin (10 mM) attenuates IL-1beta-stimulated iNOS expression after culture for 24 and 48 hours. Conclusion(s): Aspirin significantly inhibits the IL-1beta-stimulated expression of iNOS, NO, and PGE2 in ovarian dispersates cultured in vitro.