Section 11
Chapter 10,229

Barley leaf Rubisco, Rubisco binding protein and Rubisco activase and their protein/protein interactions

Demirevska-Kepova, K.; Simova-Stoilova, L.; Kjurkchiev, S.

Bulgarian Journal of Plant Physiology 25(3-4): 31-44


Accession: 010228613

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The existence of protein/protein interactions between Rubisco (R), Rubisco binding protein (RBP) and Rubisco activase (RA) was proven independently in the presence or in the absence of the ATP-regenerating system. Sandwich and inhibitory ELISA, immunoprecipitation and immunoblotting methods were used with monoclonal antibodies (Mabs) against barley Rubisco or polyclonal antibodies against barley RBP or RA. The results show that the protein/protein interactions are localized on the Rubisco large subunits at a distance from the active site and from the sites connected with Mabs.

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