+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Biochemical and genetic evidence of enterocin P production by two Enterococcus faecium-like strains isolated from fermented sausages



Biochemical and genetic evidence of enterocin P production by two Enterococcus faecium-like strains isolated from fermented sausages



Current Microbiology 39(5): 282-290



Two bacteriocin-producing Enterococcus faecium-like strains were independently isolated from fermented sausages. Bacteriocins were purified to homogeneity by ammonium sulfate precipitation, gel filtration, cationic exchange, hydrophobic interaction, and reverse-phase liquid chromatography. Two peptide inhibitory fractions were purified from each strain, denominated A and B for E. faecium AA13, and C and D for E. faecium G16. Fraction B was blocked for amino acid sequencing by Edman degradation, while the amino acid sequences obtained from peptides A, C, and D contained the YGNGV consensus motif in positions 5 to 9, and the ATRS sequence in positions 1 to 4. By use of PCR techniques and nucleotide sequencing, the structural gene of enterocin P was found both in E. faecium AA13 and E. faecium G16. Metabolic and genetic features of the two strains suggest that they are slightly different, they may produce more than one bacteriocin, and both produce enterocin P.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 010239447

Download citation: RISBibTeXText

PMID: 10489438


Related references

Biochemical and Genetic Evidence of Enterocin P Production by TwoEnterococcus faecium-Like Strains Isolated from Fermented Sausages. Current Microbiology 39(5): 282-290, 1999

Enterococcus faecium P21: A strain occurring naturally in dry-fermented sausages producing the class II bacteriocins enterocin A and enterocin B. Food Microbiology 18(2): 115-131, 2001

Biochemical and genetic evidence that Enterococcus faecium L50 produces enterocins L50A and L50B, the sec-dependent enterocin P, and a novel bacteriocin secreted without an N-terminal extension termed enterocin Q. Journal of Bacteriology 182(23): 6806-6814, 2000

Complete sequence of the enterocin Q-encoding plasmid pCIZ2 from the multiple bacteriocin producer Enterococcus faecium L50 and genetic characterization of enterocin Q production and immunity. Applied and Environmental Microbiology 72(10): 6653-6666, 2006

Immunochemical characterization of temperature-regulated production of enterocin L50 (EntL50A and EntL50B), enterocin P, and enterocin Q by Enterococcus faecium L50. Applied and Environmental Microbiology 72(12): 7634-7643, 2006

Concomitant production of lipase, protease and enterocin by Enterococcus faecium NCIM5363 and Enterococcus durans NCIM5427 isolated from fish processing waste. International Aquatic Research 4(1), 2012

Enterocin AS-48RJ: a variant of enterocin AS-48 chromosomally encoded by Enterococcus faecium RJ16 isolated from food. Systematic and Applied Microbiology 28(5): 383-397, 2005

Biochemical and genetic characterization of enterocin P, a novel sec-dependent bacteriocin from Enterococcus faecium P13 with a broad antimicrobial spectrum. Applied and Environmental Microbiology 63(11): 4321-4330, 1997

Biochemical and genetic characterization of enterocin A from Enterococcus faecium, a new antilisterial bacteriocin in the pediocin family of bacteriocins. Applied and Environmental Microbiology 62(5): 1676-1682, 1996

Atypical genetic locus associated with constitutive production of enterocin B by Enterococcus faecium BFE 900. Applied and Environmental Microbiology 65(5): 2170-2178, 1999

Biochemical and genetic evidence for production of enterocins A and B by Enterococcus faecium WHE 81. International journal of food microbiology 70(3): 291-301, 2001

Biochemical and genetic evidence for production of enterocins A and B by Enterococcus faecium WHE 81. International Journal of Food Microbiology 70(3): 291-301, 2001

Single nucleotide polymorphism analysis of the enterocin P structural gene of Enterococcus faecium strains isolated from nonfermented animal foods. Molecular Nutrition and Food Research 50(12): 1229-1238, 2006

Genetic identification of the bacteriocins produced by Enterococcus faecium IT62 and evidence that bacteriocin 32 is identical to enterocin IT. Antimicrobial Agents and ChemoTherapy 53(5): 1907-1911, 2009

Production of enterocin A by Enterococcus faecium MMRA isolated from 'Rayeb', a traditional Tunisian dairy beverage. Journal of Applied Microbiology 108(5): 1685-1693, 2010