EurekaMag
+ Translate
+ Most Popular
Gaucher's disease;thirty-two years experience at Siriraj Hospital
A study of Macrobathra Meyrick from China (Lepidoptera, Cosmopterigidae)
First occurrence in ores of tetragonal chalcocite
Effects of trace element nutrition on sleep patterns in adult women
N.Z. range management guidelines. 2. Design of grazing management systems for tussock country
A case of lipoma of the esophagus
A revision of world Acanthosomatidae (Heteroptera: Pentatomidae): keys to and descriptions of subfamilies, tribes and genera, with designation of types
Life history of the coronate scyphozoan Linuche unguiculata (Swartz, 1788)
Perceptual restoration of obliterated sounds
Mutagenicity studies on two chromium(III) coordination compounds
The formation of the skeleton. I. Growth of a long bone. 1st appearance of a center of calcification
Leucopenia and abnormal liver function in travellers on malaria chemoprophylaxis
The joint commission: four key root causes loom large in sentinel event data
Treatment of vitiligo with topical 15% lactic acid solution in combination with ultra violet-A
Behaviour of dairy cows within three hours after feed supply: I. Influence of housing type and time elapsing after feed supply
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Management and control of patients with type 2 diabetes mellitus in Lebanon: results from the International Diabetes Management Practices Study (IDMPS)
The diet composition and nutritional knowledge of patients with anorexia nervosa
Physoporella croatica Herak, 1958 of the Slovak karst Anisian (Slovakia, the West Carpathians Mts.)
Bright lights, big noise. How effective are vehicle warning systems?
Ein Plesiosaurier-Rest mit Magensteinen aus mittlerem Lias von Quedlinburg
Incidence of Chlamydia trachomatis in patients with sterility
Monster soup: the microscope and Victorian fantasy
Preliminary tests with residual sprays against poultry lice
Duration of the life of plants in phylogeny

Changes in mRNA expression induced by sustained noradrenaline stimulation are different for alpha1-adrenoceptor subtypes in HEK293 cells


Changes in mRNA expression induced by sustained noradrenaline stimulation are different for alpha1-adrenoceptor subtypes in HEK293 cells



Clinical and Experimental Pharmacology and Physiology 29(12): 1084-1090



ISSN/ISBN: 0305-1870

PMID: 12390296

DOI: 10.1046/j.1440-1681.2002.03775.x

1. The aim of the present study was to investigate noradrenaline (NA)-induced regulation of alpha1-adrenoceptor (AR) mRNA expression in human embryonic kidney (HEK) 293 cells stably expressing cloned alpha1-AR subtypes with similar receptor densities. Stable transfection was performed by calcium phosphate precipitation. Receptor expression was detected by radioligand binding assay. The mRNA expression was measured by RNase protection assay. 2. alpha1-Adrenoceptor subtype mRNA respond in distinct ways following prolonged exposure to NA. The mRNA level of the alpha 1A-AR subtype was unchanged, the mRNA level of the alpha 1B-AR subtype was increased and the mRNA level of the alpha 1D-AR subtype declined time dependently. The protein kinase C (PKC) inhibitor calphostin C or RO 31-8220 abolished the NA-induced downregulation of alpha 1D-AR mRNA. Phorbol myristate acetate (PMA), a PKC activator, similarly repressed the effects of NA on alpha 1D-AR. However, calphostin C, RO 31-8220 or PMA had no effect on the induction of alpha 1B-AR mRNA by NA. The Ca2+-ATPase inhibitor thapsigargin or the calcium chelator 1,2-bis-(o-aminophenoxy) ethane-N,N,N',N'-tetraacetic acid tetra (acetoxymethyl) ester (BAPTA/AM) had no effect on the repression of alpha 1D-AR mRNA, but did inhibit the induction of alpha 1B-AR mRNA by NA. Noradrenaline significantly decelerated the degradation of alpha 1B-AR mRNA, but had no effect on the degradation of alpha 1D-AR mRNA. 3. Thus, the mRNA expression of three alpha1-AR subtypes in HEK293 cells is differentially regulated through distinct signal transduction pathways under sustained NA stimulation. The upregulation of alpha 1B-AR mRNA is via the Ca2+ pathway, whereas the downregulation of alpha 1D-AR mRNA is via the PKC pathway.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 010294316

Download citation: RISBibTeXText

Related references

Different susceptibilities to desensitization of three alpha 1-adrenoceptor subtypes induced by sustained norepinephrine stimulation. Sheng Li Xue Bao: 49(1): 1-6, 1997

Sustained norepinephrine stimulation induces different regulation of expression in three a1-adrenoceptor subtypes. Life Sciences 69(3): 1-8, 2001

Sustained norepinephrine stimulation induces different regulation of expression in three alpha1-adrenoceptor subtypes. Life Sciences 69(3): 301-308, 2001

Heterogeneity of alpha-1-adrenoceptor subtypes involved in noradrenaline-induced contractions of dog blood vessels. Japanese Journal of Pharmacology 61(Suppl 1): 258P, 1993

Alpha-1-Adrenoceptor subtypes involved in noradrenaline- and oxymetazoline-induced contractions of rat thoracic aorta. Japanese Journal of Pharmacology 71(Suppl 1): 235P, 1996

Effect of alpha-1-adrenoceptor stimulation on gene expression for alpha-AR subtypes, alpha-actin and growth of arterial and venous smooth muscle. FASEB Journal 7(3-4): A881, 1993

Heterogeneity of alpha 1-adrenoceptor subtypes mediating noradrenaline-induced contractions of the rat superior mesenteric artery. Pharmacology 56(4): 196-206, 1998

Relation between alpha 1-adrenoceptor subtypes and noradrenaline-induced contraction in rat portal vein smooth muscle. British Journal of Pharmacology 110(1): 207-212, 1993

Alpha-1 adrenoceptor subtypes involved in noradrenaline-induced contraction of snake (Bothrops jararaca) isolated aorta. Comparative Biochemistry and Physiology C Pharmacology Toxicology and Endocrinology 111(2): 151-156, 1995

Functional characterisation of alpha 1-adrenoceptor subtypes mediating noradrenaline-induced inositol phosphate formation in rat thalamus slices. European Journal of Pharmacology 318(1): 175-184, 1996

Alpha 1-adrenoceptor subtypes mediating noradrenaline-induced contraction of pulmonary artery from pulmonary hypertensive rats. European Journal of Pharmacology 482(1-3): 255-263, 2003

Alpha-1A- and alpha-1B-adrenoceptor subtypes and contractions of the rat thoracic aorta to noradrenaline, phenylephrine and oxymetazoline. British Journal of Pharmacology 112(Proceedings Suppl MAY): 55P, 1994

Positive inotropic and chronotropic effects of noradrenaline which cannot by explained by alpha adrenoceptor or beta adrenoceptor stimulation. British Journal of Pharmacology 86(Suppl): 699P, 1985

A quantitative analysis of mRNA expression of alpha 1 and beta-adrenoceptor subtypes and their functional roles in human normal and obstructed bladders. Journal of Urology 170(2 Pt 1): 649-653, 2003

Noradrenaline-induced contraction of human saphenous vein and human internal mammary artery: involvement of different alpha-adrenoceptor subtypes. Naunyn-Schmiedeberg's Archives of Pharmacology 366(2): 104-109, 2002