Characterization of the Streptomyces coelicolor A3 (2) wblE gene, encoding a homologue of the sporulation transcription factor
Homerová, D.; Sevcíková, J.; Kormanec, J.
Folia Microbiologica 48(4): 489-495
ISSN/ISBN: 0015-5632 PMID: 14533480 DOI: 10.1007/bf02931330
The gene corresponding to the recently identified whiB-paralogous gene wblE in S. coelicolor was found after sequencing the downstream region of the stress-response sporulation-specific sigma-factor gene, sigH, in S. coelicolor A3(2). Sequence analysis has revealed an ORF exhibiting high similarity to sporulation transcription factors WhiB and WhiD. A stable null mutant of the wblE gene was obtained by integrative transformation, via double cross-over.