+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Chiral separation of methoxamine and lobeline in capillary zone electrophoresis using ethylbenzene-deactivated fused-silica capillary columns and cyclodextrins as buffer additives



Chiral separation of methoxamine and lobeline in capillary zone electrophoresis using ethylbenzene-deactivated fused-silica capillary columns and cyclodextrins as buffer additives



Journal of Pharmaceutical and Biomedical Analysis 29(6): 999-1003, 1 August



The complete chiral separation of methoxamine and lobeline was achieved by capillary zone electrophoresis on an ethylbenzene-deactivated fused-silica capillary column and with cyclodextrins (CDs) as buffer additives. Among the CDs investigated in this study, i.e. alpha-CD, beta-CD, dimethyl-beta-CD, hydroxypropyl-beta-CD and gamma-CD, all the three beta-type CDs showed chiral recognition on the two drugs investigated. Under the investigated conditions, the baseline chiral separation of methoxamine can be achieved with 90 mM Tris-H3PO4 (pH 2.5) containing 11.5 mM of the three beta-type CDs, with dimethyl-beta-CD giving the best resolution, whereas the baseline chiral separation of lobeline can be realized by using 90 mM Tris-H3PO4 buffer (pH 2.5) containing 5.8 mM dimethyl-beta-CD or 29.5 mM hydroxypropyl-beta-CD.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 010315872

Download citation: RISBibTeXText

PMID: 12110384

DOI: 10.1016/s0731-7085(02)00140-1


Related references

Chiral separation of lorazepam on ovomucoid-bonded columns Peak coalescence due to racemization. Journal Of Liquid Chromatography. 16(4): 879-891, 1993

Chiral separation of homocysteine by derivatization with 4-aminosulfonyl-7-fluoro-2,1,3-benzoxadiazole followed by capillary electrophoresis using gamma-cyclodextrin. Journal of Chromatography. A 877(1-2): 217-223, 2000

Cyclodextrins as a versatile chiral recognition reagents for use in a variety of optical and separative analyses. Journal of Pharmaceutical and Biomedical Analysis 12(1): 1-3, 1994

Chiral separations of beta-blocking drug substances using chiral stationary phases. Journal of Chromatography A 708(2): 253-261, 1995

Improved capillary electrophoretic separation of glycosylated oligopeptides through addition of poly , and analysis by electrospray mass spectrometry. Journal of Chromatography A 805(1-2): 285-293, 1998

Enantioseparation of tetramisole by capillary electrophoresis and high performance liquid chromatography and application of these techniques to enantiomeric purity determination of a veterinary drug formulation of L-levamisole. Journal of Separation Science 25(12): 733-740, 2002

Enantioseparation of 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate-derivatized amino acids by capillary zone electrophoresis using native and substituted beta-cyclodextrins as chiral additives: II. Evaluation of complexation constants. Journal of Chromatography A 759(1-2): 167-175, 1997

Chiral separation of lorazepam on ovomucoid-bonded columns: Peak coalescence due to racemization. Journal of Liquid Chromatography 16(4): 879-891, 1993

Chiral separation of halogenated amino acids by ligand-exchange capillary electrophoresis. Electrophoresis 26(20): 3878-3883, 2005

Separation of chiral compounds with alpha acid glycoprotein as selector. Atti della Societa dei Naturalisti e Matematici di Modena 121: 111-120, 1990

Use of cyclodextrins and their derivatives for chiral analysis by high field nuclear magnetic resonance spectroscopy. Analytical Proceedings 29(6): 229-231, 1992

Chiral separation of a pharmacuetical intermediate by a simulated moving bed process. Journal of Chromatography A 832(1-2): 55-65, 1999

Chiral separation of hesperidin and naringin and its analysis in a butanol extract of Launeae arborescens. Natural Product Research 24(7): 669-681, 2010

Capillary zone electrophoresis of fish muscle sarcoplasmic protein. Journal of Food Science 59(6): 1267-1270, 1994

Comparison of sulfate and nitrate particle mass concentrations measured by IMPROVE and the CDN. Atmospheric Environment 35(5): 905-916, 2001