Chloroplast biogenesis 81: Transient formation of divinyl chlorophyll a following a 2.5 ms light flash treatment of etiolated cucumber cotyledons

Adra, A.N.; Rebeiz, C.A.

Photochemistry and Photobiology 68(6): 852-856

1998


ISSN/ISBN: 0031-8655
DOI: 10.1111/j.1751-1097.1998.tb05295.x
Accession: 010317075

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Abstract
The possible conversion of nascent divinyl (DV) chlorophyllide a (Chlide alpha) to DV chlorophyll a (Chl alpha during the early stages of greening in a dark divinyl-light divinyl-light/dark divinyl (DDV-LDV-LDDV) plant species was investigated. Etiolated cucumber cotyledons (Cucumis sativus L.) were subjected to a 2.5 ms light flash followed by darkness. The DV and monovinyl (MV) components of the protochlorophyllide a (Pchlide alpha), Chlide a, Pchlide a ester and Chl alpha pools were monitored quantitatively by high-resolution spectrofluorometry, immediately following the light treatments and after various periods in darkness. The light treatment photoconverted DV and MV Pchlide a to DV and MV Chlide alpha. Some photoconversion of MV Pchlide a ester to MV Chl a also appeared to take place. A sharp rise in the level of DV Chl alpha following the light treatment could not be accounted for by photoconversion of DV Pchlide a ester. It must have arisen by rapid esterification of nascent DV Chlide alpha. After illumination, the level of DV Chl alpha rose for 5 s and then declined. The implications of the transient rise and fall of DV Chl alpha content following illumination to the Chl a biosynthetic heterogeneity is discussed.