+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

Cloning and expression of the insecticidal crystal protein gene Cry1Ca9 of Bacillus thuringiensis G10-01A from Taiwan granaries

Cloning and expression of the insecticidal crystal protein gene Cry1Ca9 of Bacillus thuringiensis G10-01A from Taiwan granaries

Current Microbiology 47(4): 295-299

ISSN/ISBN: 0343-8651

PMID: 14629010

DOI: 10.1007/s00284-002-3982-4

A new cry gene (cry1Ca9) was cloned and sequenced from a Bacillus thuringiensis isolate native to Taiwan (G10-01A). The cry1C-type gene, designated cry1Ca9, consisted of an open reading frame of 3,567 bp, encoding a protein of 1,189 amino acid residues. The polypeptide has the deduced amino acid sequences predicting molecular masses of 134.7 kDa. The gene sequence was compared against the GenBank nucleotide sequence data base. It was found that the cry1Ca9 gene coded for a 134.7-kDa protoxin which had greater than 99.8% homology with the previously reported cry1Ca1 gene, as only three mismatches were found between the two amino acid sequences. When the Cry1Ca9 toxin was expressed in a crystal-negative strain of B. thuringiensis (cryB-), elliptical crystals were produced. Cell extracts from this recombinant strain appear to have high insecticidal activity against lepidopteran larvae (Plutella xylostella).

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 010336297

Download citation: RISBibTeXText

Related references

Cloning and expression of the insecticidal crystal protein gene, cry1Ac5, of Bacillus thuringiensis D4-01 from Taiwan. Plant Protection Bulletin Taipei 48(1): 17-30, 2006

Cloning and expression in Escherichia coli of an insecticidal crystal protein gene from Bacillus thuringiensis var. aizawai HD-133. Journal of General Microbiology 133(10): 2921-2931, 1987

Cloning and expression of cryIVD gene of insecticidal crystal protein of Bacillus thuringiensis in the acrystalliferous strain. Entomologia Sinica 2(3): 239-246, 1995

Cloning and expression of an insecticidal crystal protein CryIIA gene from Bacillus thuringiensis subsp. kurstaki HD-1. Korean Journal of Applied Entomology 32(3): 300-306, 1993

Cloning and Expression of an Insecticidal k-73 Type Crystal Protein Gene from Bacillus thuringiensis var. kurstaki into Escherichia coli. Applied and Environmental Microbiology 50(3): 623-628, 1985

Cloning and characterization analysis of insecticidal crystal protein gene cry1A from Bacillus thuringiensis. Southwest China Journal of Agricultural Sciences 17(1): 38-40, 2004

Cloning and characterization of an insecticidal crystal protein gene from Bacillus thuringiensis subspecies kenyae. Journal of Genetics 81(1): 5-11, 2002

Molecular cloning and characterization of the insecticidal crystal protein gene of Bacillus thuringiensis var. tenebrionis. Proceedings of the National Academy of Sciences of the United States of America 84(20): 7036-7040, 1987

The insecticidal crystal protein Cry2Ab10 from Bacillus thuringiensis: cloning, expression, and structure simulation. Biotechnology Letters 30(3): 513-519, 2008

Cloning and partial characterization of the bacillus thuringiensis dendrolimus t84a1 insecticidal crystal protein gene. Journal of the Faculty of Applied Biological Science Hiroshima University 28(1-2): 63-70, 1989

Characterization of insecticidal crystal protein cry gene of Bacillus thuringiensis from soil of Sichuan Basin and cloning of novel holotype cry gene. Wei Sheng Wu Xue Bao 49(3): 324-330, 2009

Screening of Bacillus thuringiensis strain with broadspectrum and high toxicity and cloning of its insecticidal crystal protein gene. Journal of South China Agricultural University 26(4): 40-42, 2005

Molecular cloning and expression of Bacillus thuringiensis subsp. galleriae insecticidal crystal protein genes in Escherichia coli. Science in China. Series B Chemistry Life Sciences and Earth Sciences 32(7): 830-836, 1989

Expression of Bacillus thuringiensis (B.t.) insecticidal crystal protein gene in transgenic potato. Botanical Bulletin of Academia Sinica 37(1): 17-23, 1996

Expression of a Bacillus thuringiensis insecticidal crystal protein gene in tobacco plants. UCLA symposia on molecular and cellular biology 8(48): 345-353, 1987