Column-switching chromatographic determination of itraconazole and its metabolite hydroxy-itraconazole in human serum

Kubalec, P.; Brandsteterová, E.

Die Pharmazie 56(5): 397-400


ISSN/ISBN: 0031-7144
PMID: 11400556
Accession: 010345367

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A column-switching HPLC assay is described for the determination of the antifungal agent itraconazole and its main metabolite hydroxy-itraconazole in serum samples. Three precolumns packed with alkyl-diol silica sorbents differing in alkyl chain length are compared in the sample clean-up step. Chromatographic separation is achieved with a Symmetry C8 column. The assay presented shows a robust and selective analytical procedure with low requirements for sample quantity, no manual sample treatment and high sample throughput.