+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Combined cervical swab and urine specimens for PCR diagnosis of genital Chlamydia trachomatis infection



Combined cervical swab and urine specimens for PCR diagnosis of genital Chlamydia trachomatis infection



Sexually Transmitted Infections 76(3): 177-178



Objectives: Sampling of both the cervix and urine increases the chance of detection of Chlamydia trachomatis compared with sampling either site alone. We determined the effect of combining urine and cervical swab specimens in the clinic setting on the sensitivity of C trachomatis polymerase chain reaction (PCR) testing. Methods: For each of 100 women attending a genitourinary medicine clinic with high likelihood of genital C trachomaris infection, one endocervical swab was placed in transport medium and another in one of two aliquots of first void urine. Four PCR assays per patient (urine + swab, swab alone, and urine alone both pre- and post-freeze-thawing) were processed by automated C trachomatis PCR (Cobas, Amplicor). An inhibition control was included with each assay to identify specimens containing PCR inhibitors. Results: 71% of women were Amplicor C trachomatis PCR positive (according to the results of at least one specimen). PCR test results were concordant for 95/100 patients, and of the five discordant result sets there was only one major discrepancy. Inhibitors of PCR were present in 22/400 specimens from 20 patients, and 16/22 were cervical swabs (p<0.001). Conclusions: Combining a cervical swab with a urine specimen is acceptable for PCR testing for genital C trachomatis infection, and has the potential to increase further the cost effectiveness of DNA based screening for C trachomatis genital infection.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 010346456

Download citation: RISBibTeXText

PMID: 10961193

DOI: 10.1136/sti.76.3.177


Related references

Combined cervical swab and urine specimen testing for PCR diagnosis of genital Chlamydia trachomatis infection. Abstracts of the Interscience Conference on Antimicrobial Agents & Chemotherapy 38: 136-137, 1998

Comparison of VIDAS PROBE with LCx for the detection of Chlamydia trachomatis in urine and genital swab specimens from females and males. Abstracts of the General Meeting of the American Society for Microbiology 100: 224, 2000

Detection of Chlamydia trachomatis and Neisseria gonorrhoeae in pooled urine and cervical swab specimens by BD probe Tec ET. International Journal of STD & AIDS 12(Suppl. 2): 115, 2001

Detection of Chlamydia trachomatis in urine samples by nucleic acid tests: comparison with culture and enzyme immunoassay of genital swab specimens. Journal of Clinical Microbiology 35(12): 3355-3357, 1997

Evaluation of the Abbott LCx ligase chain reaction assay for detection of Chlamydia trachomatis and Neisseria gonorrhoeae in urine and genital swab specimens from a sexually transmitted disease clinic population. Journal of Clinical Microbiology 36(6): 1630-1633, 1998

Evaluation of self-collected vaginal swab, first void urine, and endocervical swab specimens for the detection of Chlamydia trachomatis and Neisseria gonorrhoeae in adolescent females. Journal of Pediatric and Adolescent Gynecology 21(6): 355-360, 2008

Comparison of polymerase chain reaction and enzyme immuno-assay in the diagnosis of Chlamydia trachomatis in urogenital swab and urine specimens. Clinical Chemistry & Laboratory Medicine 37(SPEC Suppl. ): S486, 1999

Evaluation of laparoscopy and endocervical swab in the diagnosis of Chlamydia trachomatis infection of the female genital tract. Archives of Gynecology and Obstetrics 253(1): 5-7, 1993

Evaluation of laparoscopy and endocervical swab in diagnosis of Chlamydia trachomatis infection of the female genital tract. Archives of Gynecology & Obstetrics 253(1): 5-7, 1993

Evaluation of a new amplified enzyme immunoassay (EIA) for the detection of Chlamydia trachomatis in male urine, female endocervical swab, and patient obtained vaginal swab specimens. Journal of Clinical Pathology 53(5): 350-354, 2000

Multiple site evaluation of the GEN-PROBE Amplified Chlamydia Trachomatis assay for detection of C trachomatis in male urethral swab and urine specimens. Abstracts of the General Meeting of the American Society for Microbiology 97: 173, 1997

Comparison of first-voided urine specimens with endocervical swab specimens for enzyme-linked immunosorbent assay detection of Chlamydia trachomatis in women. Archives of Family Medicine 3(8): 672-675, 1994

A comparison of first-void urine, self-administered low vaginal swab, self-inserted tampon, and endocervical swab using PCR tests for the detection of infection with Chlamydia trachomatis. Sexual Health 1(1): 51-54, 2004

Screening for Chlamydia trachomatis and Mycoplasma genitalium; is first void urine or genital swab best?. Sexually Transmitted Infections 91(2): 141, 2015

Evaluation of diagnostic efficacy of PCR methods for Chlamydia trachomatis infection in genital and urine specimens of symptomatic men and women in India. Japanese Journal of Infectious Diseases 56(3): 88-92, 2003