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Comparative study of six kits of polymerase chain reaction assay with cell culture and ligase chain reaction assay in detection of C. trachomatis infection

Comparative study of six kits of polymerase chain reaction assay with cell culture and ligase chain reaction assay in detection of C. trachomatis infection

Zhonghua Pifuke Zazhi 34(3): 181-183

Objective: To evaluate the diagnostic value of six home made PCR kits for the detection of Chlamydia trachomatis in patients with sexually transmitted diseases, cell culture and LCR were used as references. Methods: Endocervical or urethral swab specimens were collected from 673 patients attending STD clinics in Beijing, Shanghai, Nanjing and Tianjin. C. trachomatis culture and PCR were performed with specimens from all patients while LCR was performed only with specimens which the culture and PCR gave discrepant results. Results: Among 616 eligible patients thirty-nine (6.3%) cases were culture positive, and PCRs revealed positive rates from 23.5% to 28.7%. In comparison with cell culture, the sensitivity of PCRs were 90% or higher. In 200 cases which the culture and PCR gave discrepant results, LCR and PCR showed excellent consistency (YI index: 0.523apprx0.881), the sensitivity and specificity of PCRs ranged from 83.9%apprx98.6% and 66.7%apprx94.7% respectively, while PCR2 showed the highest YI index (0.881). With an expanded gold standard for discrepant results, we found that the specificity and sensitivity of PCRs were higher than 95% and 85%, respectively. Conclusions: Domestic produced PCR kits for Chlamydia trachomatis detection are highly sensitive and specific, however, the quality control of laboratory still remains important in the clinical application.

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