Comparison of different pretreatments in ethanol fermentation using corn cob hemicellulosic hydrolysate with Pichia stipitis and Candida shehatae
Eken Saracoglu, N.; Arslan, Y.
Biotechnology Letters 22(10): 855-858
ISSN/ISBN: 0141-5492 DOI: 10.1023/a:1005663313597
A hemicellulosic hydrolysate was prepared with 0.3 M H2SO4 at 98 degreeC for 1 h. The total initial reducing sugar was maintained at 45 g l-1 by synthetic xylose supplementation. The seven detoxification methods were employed including either the single addition of solid CaO (to pH 10 or 6) or its combinations with zeolite shaking. Over-liming gave the hydrolysate that was most completely fermented by Pichia stipitis and Candida shehatae at 30 degreeC, pH 6, among the tested methods.