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Comparison of high performance liquid chromatography and bioassay for determination of ofloxacin in human plasma



Comparison of high performance liquid chromatography and bioassay for determination of ofloxacin in human plasma



Zhongguo Xinyao yu Linchuang Zazhi 22(4): 201-204



AIM: To compare high performance liquid chromatography (HPLC) and bioassay for determination of ofloxacin in human plasma. METHODS: Ofloxacin injections were given to eight healthy volunteers with a single dose, iv, drip, 200 mg. Plasma concentrations were determined by HPLC method and bioassay, respectively. RESULTS: The calibration curve of HPLC method was linear in the range of 0.125-5 mgcntdotL-1. The recovery of HPLC was within (93+-s 5)% and (106+-7)%, within-day and day-to-day RSD were 3.9%-7.4% and 3.0%-7.7%. The calibration curve of bioassay was linear in the range of 0.125-5 mgcntdotL-1. The recovery of bioassay was within (99+-3)% and (107+-6)%, within-day and day-to-day RSD were 2.8%-3.9% and 3.4%-8.0%. There was no significant difference in ofloxacin mean plasma concentrations and main pharmacokinetic parameters obtained by HPLC method and the bioassay. CONCLUSION: The recovery and the precision of the both methods were found to be similar, therefore both can be used to determinate plasma concentrations of ofloxacin.

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