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Construction and evaluation of a capillary array DNA sequencer based on a micromachined sheath-flow cuvette

Construction and evaluation of a capillary array DNA sequencer based on a micromachined sheath-flow cuvette

Electrophoresis 21(7): 1329-1335

A capillary array electrophoresis DNA sequencer is reported based on a micromachined sheath-flow cuvette as the detection chamber. This cuvette is equipped with a set of micromachined features that hold the capillaries in precise registration to ensure uniform spacing between the capillaries, in order to generate uniform hydrodynamic flow in the cuvette. A laser beam excites all of the samples simultaneously, and a microscope objective images fluorescence onto a set of avalanche photodiodes, which operate in the analog mode. A high-gain transimpedance amplifier is used for each photodiode, providing high duty-cycle detection of fluorescence.

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Accession: 010378098

Download citation: RISBibTeXText

PMID: 10826677

DOI: 10.1002/(SICI)1522-2683(20000401)21:7<1329::AID-ELPS1329>3.0.CO;2-2

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