+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Construction of a genetically engineered microorganism for CO2 fixation using a Rhodopseudomonas/Escherichia coli shuttle vector



Construction of a genetically engineered microorganism for CO2 fixation using a Rhodopseudomonas/Escherichia coli shuttle vector



FEMS Microbiology Letters 225(1): 69-73, 8 August



The CO2 fixation ability of Rhodopseudomonas palustris DH was enhanced by introducing the recombinant plasmid pMG-CBBM containing the form II ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO) gene (cbbM) isolated from Rps. palustris NO. 7. Sequencing of a 3.0-kb PstI fragment containing the cbbM gene revealed an open reading frame encoding 461 amino acids, homologous to known cbbM genes, with a ribosome binding site upstream of cbbM and a terminator downstream of cbbM, without promoter. pMG-CBBM, a Rhodopseudomonas/Escherichia coli shuttle expression plasmid, was derived from the Rhodopseudomonas/E. coli shuttle cloning vector pMG105, by inserting the promoter of the pckA gene and the cbbM gene into its multiple cloning site. Plasmid pMG-CBBM was transformed into Rps. palustris DH by electroporation, and was stably maintained when transformants were grown either photoheterotrophically or photolithoautotrophically in the absence of antibiotics. This is the first report of an expression plasmid containing a Rps. palustris-specific promoter that allows stable expression of a foreign gene in the absence of antibiotic selection.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 010378394

Download citation: RISBibTeXText

PMID: 12900023

DOI: 10.1016/s0378-1097(03)00482-8


Related references

Bioaugmentation of the decolorization rate of acid red GR by genetically engineered microorganism Escherichia coli JM109 pGEX-AZR. World Journal of Microbiology and Biotechnology 24(1): 23-29, 2008

Construction of a clostridium acetobutylicum and escherichia coli shuttle vector. Biotechnology Letters 13(1): 1-6, 1991

Construction of a shuttle vector in bacillus subtilis and escherichia coli. Journal of Pharmaceutical Sciences 76(11): S184, 1987

The construction of shuttle plasmid vector of streptomyces and escherichia coli. Acta Genetica Sinica 17(4): 327-334, 1990

Construction of a shuttle vector for streptomyces and escherichia coli system. Szabo, G , S Biro And M Goodfellow (Ed ) Symposia Biologica Hungarica, Vol 32 Biological, Biochemical And Biomedical Aspects Of Actinomycetes, Parts A And B; Sixth International Symposium on Actinomycetes Biology, Debrecen, Hungary, August 26-30, 1985 Xxviii+429p (Part A); Xxii+454p (Part B) Akademiai Kiado: Budapest, Hungary Illus 145, 1986, 1987

Construction of a shuttle vector for use between Pasteurella multocida and Escherichia coli. Plasmid 30(3): 268-273, 1993

Construction and characterization of a Mycobacterium-Escherichia coli shuttle vector. Plasmid 25(2): 149-153, 1991

Construction of a shuttle vector between escherichia coli and zymomonas anaerobia. Biotechnology Letters 9(3): 163-168, 1987

Construction of a shuttle vector of Helicobacter pylori and Escherichia coli. Journal of the Korean Society for Microbiology 31(5): 557-564, 1996

Construction of new shuttle vector for escherichia coli and bacillus subtilis. Korean Journal of Genetics 10(1): 53-63, 1988

Construction of a shuttle vector between neisseria gonorrhoeae and escherichia coli. Abstracts of the Annual Meeting of the American Society for Microbiology 83: D75, 1983

Construction of shuttle vector plasmid between Clostridium acetobutylicum and Escherichia coli. Agricultural and Biological Chemistry 54(2): 437-441, 1990

Construction and characterization of a Pasteurella- Actinobacillus-Escherichia coli shuttle vector. Donachie, W, Lainson, F A, Hodgson, J C Haemophilus, Actinobacillus, and Pasteurella: 210, 1995