+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Continuous loss of oocytes throughout meiotic prophase in the normal mouse ovary

Continuous loss of oocytes throughout meiotic prophase in the normal mouse ovary

Developmental Biology 258(2): 334-348

The number of germ cells reaches the maximum just prior to entry into meiosis, yet decreases dramatically by a few days after birth in the female mouse, rat, and human. Previous studies have reported a major loss at the pachytene stage of meiotic prophase during fetal development, leading to the hypothesis that chromosomal pairing abnormalities may be a signal for oocyte death. However, the identification as well as the quantification of germ cells in these studies have been questioned. A recent study using Mouse Vasa Homologue (MVH) as a germ cell marker reached a contradictory conclusion claiming that oocyte loss occurs in the mouse only after birth. In the present study, we established a new method to quantify murine germ cells by using Germ Cell Nuclear Antigen-1 (GCNA-1) as a germ cell marker. Comparison of GCNA-1 and MVH immunolabeling revealed that the two markers identify the same population of germ cells. However, nuclear labeling of GCNA-1 was better suited for counting germ cells in histological sections as well as for double labeling with the antibody against synaptonemal complex (SC) proteins in chromosome spreading preparations. The latter experiment demonstrated that the majority of GCNA-1-labeled cells entered and progressed through meiotic prophase during fetal development. The number of GCNA-1-positive cells in the ovary was estimated by counting the labeled cells retained in chromosome spreading preparations and also in histological sections by using the ratio estimation method. Both methods demonstrated a continuous decline in the number of GCNA-1-labeled cells during fetal development when the oocytes progress through meiotic prophase. These observations suggest that multiple causes are responsible for oocyte elimination.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 010380590

Download citation: RISBibTeXText

PMID: 12798292

DOI: 10.1016/s0012-1606(03)00132-5

Related references

Onset and progress of meiotic prophase in the oocytes in the B6.YTIR sex-reversed mouse ovary. Biology of Reproduction 69(6): 1879-1889, 2003

Cyclic AMP in oocytes controls meiotic prophase I and primordial folliculogenesis in the perinatal mouse ovary. Development 142(2): 343-351, 2015

Progress of meiotic prophase and apoptotic cell death in oocytes in the XY sex-reversed fetal mouse ovary. Biology of Reproduction 62(Suppl. 1): 174, 2000

Apoptosis in mouse fetal and neonatal oocytes during meiotic prophase one. Bmc Developmental Biology 7: 87, 2007

Synapsis synaptic adjustment and dna synthesis in meiotic prophase of mouse oocytes. Journal of Cell Biology 95(2 Part 2): 77A, 1982

Patterns of RNA synthesis in early meiotic prophase oocytes from fetal mouse ovaries. Chromosoma 67(1): 21-40, 1978

The behavior of the X- and Y-chromosomes in the oocyte during meiotic prophase in the B6.Y(TIR)sex-reversed mouse ovary. Reproduction 135(2): 241-252, 2008

Induction of fetal primary oocytes and the meiotic prophase from mouse pluripotent stem cells. Methods in Cell Biology 144: 409-429, 2018

Regulation of Meiotic Prophase Arrest in Mouse Oocytes by GPR3, a Constitutive Activator of the GsG Protein. Journal of Cell Biology 171(2): 255-265, 2005

Regulation of meiotic prophase arrest in mouse oocytes by GPR3, a constitutive activator of the Gs G protein. Journal of Cell Biology 171(2): 255-265, 2005

Sex-specific gene expression during meiotic prophase I Xlr , not its male homologue Xmr , is expressed in mouse oocytes. Biology of Reproduction 67(5): 1646-1652, November, 2002

An electron microscope study on the oogenesis in the mouse, with special reference to the behaviours of oogonia and oocytes at meiotic prophase. Archivum Histologicum Japonicum 25(5): 533-555, 1965

Caspase 9 is constitutively activated in mouse oocytes and plays a key role in oocyte elimination during meiotic prophase progression. Developmental Biology 377(1): 213-223, 2013

Molecular Mechanisms of Prophase I Meiotic Arrest Maintenance and Meiotic Resumption in Mammalian Oocytes. Reproductive Sciences 2018: 1933719118765974, 2018

The molecular control of meiotic chromosomal behavior: events in early meiotic prophase in Drosophila oocytes. Annual Review of Physiology 74: 425-451, 2012