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Crystallographic analysis of the MoFe protein of nitrogenase from a nifV mutant of Klebsiella pneumoniae identifies citrate as a ligand to the molybdenum of iron molybdenum cofactor (FeMoco)



Crystallographic analysis of the MoFe protein of nitrogenase from a nifV mutant of Klebsiella pneumoniae identifies citrate as a ligand to the molybdenum of iron molybdenum cofactor (FeMoco)



Journal of Biological Chemistry 277(38): 35263-6



The x-ray crystal structure of NifV- Klebsiella pneumoniae nitrogenase MoFe protein (NifV- Kp1) has been determined and refined to a resolution of 1.9 ANG. This is the first structure for a nitrogenase MoFe protein with an altered cofactor. Moreover, it is the first direct evidence that the organic acid citrate is not just present, but replaces homocitrate as a ligand to the molybdenum atom of the iron molybdenum cofactor (FeMoco).

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Accession: 010401479

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PMID: 12133839

DOI: 10.1074/jbc.M205888200



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