+ Site Statistics
References:
52,572,879
Abstracts:
28,705,754
PMIDs:
27,750,366
DOIs:
25,464,004
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Cyclic mechanical stress induces extracellular matrix degradation in cultured chondrocytes via gene expression of matrix metalloproteinases and interleukin-1



Cyclic mechanical stress induces extracellular matrix degradation in cultured chondrocytes via gene expression of matrix metalloproteinases and interleukin-1



Journal of Biochemistry 125(5): 966-975



To clarify the mechanism of cartilage degradation induced by mechanical stress, we investigated the influence of cyclic tension force (CTF) on the metabolism of cultured chondrocytes. The chondrocytes were exposed to CTF using a Flexercell strain unit. Five or 15 kPa of high frequency CTF significantly inhibited the syntheses of DNA, proteoglycan, collagen, and protein. Fifteen kPa of high frequency CTF induced the expression of interleukin-1 (IL-1), matrix metalloproteinase (MMP)-2 and -9 mRNA, and increased the production of pro- and active-MMP-9. The degradation of proteoglycan was inhibited by and MMP inhibitor, indicating that MMPs are involved in the degradation of proteoglycans induced by high frequency CTF. Moreover, reducing the frequency of CTF from high to low decreased the inhibition of proteoglycan synthesis. These findings suggest that the CTF frequency is one of the key determinants of chondrocyte metabolism. Low magnitude CTF, whether high or low frequency, did not cause the gene expression of cartilage degradation factors, suggesting that this CTF magnitude causes only minor changes in the cartilage matrix. High magnitude and frequency CTF caused the gene expression of IL-1 and MMP-9, followed by increases in the production of MMP-2 and -9 proteins, suggesting that excessive and continuous cyclic mechanical stress induces the production of IL-1 and MMP-9, resulting in cartilage degradation.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 010405150

Download citation: RISBibTeXText

PMID: 10220591

DOI: 10.1093/oxfordjournals.jbchem.a022376


Related references

Interleukin-4 downregulates the cyclic tensile stress-induced matrix metalloproteinases-13 and cathepsin B expression by rat normal chondrocytes. Acta Medica Okayama 62(2): 119-126, 2008

Cyclic AMP regulates extracellular matrix gene expression and metabolism in cultured primary rat chondrocytes. Matrix Biology 28(6): 354-364, 2010

Cyclic compressive mechanical stimulation induces sequential catabolic and anabolic gene changes in chondrocytes resulting in increased extracellular matrix accumulation. Matrix Biology 25(6): 323-331, 2006

Mechanical compression alters gene expression and extracellular matrix synthesis by chondrocytes cultured in collagen I gels. Biomaterials 23(4): 1249-1259, 2002

Nitric oxide mediates interleukin-1-induced gene expression of matrix metalloproteinases and basic fibroblast growth factor in cultured rabbit articular chondrocytes. Journal of Biochemistry 123(3): 431-439, 1998

Betulin suppressed interleukin-1β-induced gene expression, secretion and proteolytic activity of matrix metalloproteinase in cultured articular chondrocytes and production of matrix metalloproteinase in the knee joint of rat. Korean Journal of Physiology and Pharmacology 21(1): 19-26, 2017

Regulatory role of extracellular matrix components in expression of matrix metalloproteinases in cultured hepatic stellate cells. Cell Structure and Function 24(5): 255-261, 2004

Interleukin-17F affects cartilage matrix turnover by increasing the expression of collagenases and stromelysin-1 and by decreasing the expression of their inhibitors and extracellular matrix components in chondrocytes. Cytokine 56(2): 376-386, 2012

Effects of the existence of nitric oxide and the degradation of extracellular matrix on CD44 mRNA expression in cultured chondrocytes. Journal of Bone and Mineral Metabolism 16(3): 178-185, 1998

The effect of IL-1beta on the expression of matrix metalloproteinases and tissue inhibitors of matrix metalloproteinases in human chondrocytes. Life Sciences 77(25): 3210-3221, 2005

Extracellular matrix protein gene expression of bovine chondrocytes cultured on resorbable scaffolds. Biomaterials 21(23): 2427-2431, 2000

Regulation of plasminogen activation, matrix metalloproteinases and urokinase-type plasminogen activator-mediated extracellular matrix degradation in human osteosarcoma cell line MG63 by interleukin-1 alpha. Journal of Bone and Mineral Research 10(9): 1374-1384, 1995

Matrix degradation by chondrocytes cultured in alginate: IL-1 beta induces proteoglycan degradation and proMMP synthesis but does not result in collagen degradation. Osteoarthritis and Cartilage 6(5): 330-340, 1999

Extracellular matrix induces albumin gene expression in cultured rat hepatocytes. Hepatology 10(4): 636, 1989

Expression of matrix metalloproteinases, tissue inhibitors of metalloproteinases, and extracellular matrix mRna following exposure to mineral fibers and cigarette smoke in vivo. Environmental Health Perspectives 105(suppl 5): 1247-1251, 1997