+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Cytochemical and ultrastructural studies on protoplast formation from disintegrated cells of the marine alga Chaetomorpha aerea (Chlorophyta)

Cytochemical and ultrastructural studies on protoplast formation from disintegrated cells of the marine alga Chaetomorpha aerea (Chlorophyta)

European Journal of Phycology 38(3): 205-216

Regeneration of protoplasts from extruded cytoplasm and successive development of aplanospores within regenerated cells are described in the marine green alga Chaetomnorpha aerea (Dillwyn) Kutzing (Cladophorales, Cladophoraceae). Agglutination of cell organelles in seawater seemed to be mediated by a complementary lectin-carbohydrate system. Three carbohydrates - D-galactosamine, D-glucosamine and alpha-D-mannose - inhibited agglutination of cell organelles. The presence of these sugar moieties on the surface of cell organelles was verified with their complementary fluorescein isothiocyanate lectins. Agglutination assays using human erythrocytes showed the presence of lectins specific for the above sugars in the protoplasm. The fluorescent probe 1-(4-trimethylammoniumphenyl)-6-phenyl-a,3,5-hexatriene revealed that the envelope initially surrounding protoplasts was not a lipid-based cell membrane. Fluorescein diacetate staining showed esterase activity in the protoplasts from the beginning of the regeneration process, suggesting that their envelopes were intact. Enzyme digestion revealed that the enclosing envelope includes polysaccharides as essential structural components and then transforms into a polysaccharide-lipid complex, before a complete plasma membrane develops within 6 h after wounding. Electron microscopic observation suggested that new plasma membrane formed by incorporation of original cell membrane into the primary envelope. An extensive agglutination of cytoplasmic vesicles was observed in the protoplasts. New cell walls developed within 24 h after wounding. Thereafter, the cells developed aplanospores or swarmers 4-10 days after wounding. The released aplanospore divided into 8 cells, which became motile biflagellate swarmers capable of germination.

(PDF emailed within 0-6 h: $19.90)

Accession: 010407514

Download citation: RISBibTeXText

DOI: 10.1080/0967026031000136330

Related references

Cyto taxonomical studies of british marine species of chaetomorpha part 1 chaetomorpha linum and chaetomorpha aerea. Phykos 10(1-2): 127-136, 1971

Antibacterial activity of marine macro alga (Chaetomorpha aerea) collected from Vellar estuary. Environment and Ecology 24(2): 280-282, 2006

Antibacterial activity of a sulfated galactan extracted from the marine alga Chaetomorpha aerea against Staphylococcus aureus. Biotechnology and Bioprocess Engineering 16(5): 937-945, 2011

From protoplasm to swarmer: Regeneration of protoplasts from disintegrated cells of the multicellular marine green alga Microdictyon umbilicatum (Chlorophyta). Journal of Phycology 38(1): 174-183, 2002

Contribution to the knowledge of chaetomorpha aerea cladophorales chlorophyta. Darwiniana (San Isidro) 26(1-4): 71-78, 1985

Purification and characterization of a lectin, bryohealin, involved in the protoplast formation of a marine green alga Bryopsis plumosa (Chlorophyta). Journal of Phycology 42(1): 86-95, 2006

Zoo sporogenesis in the multi nucleate green alga chaetomorpha aerea. Journal of Phycology 11(SUPPL): 18, 1975

Molecular characterization of the lectin, bryohealin, involved in protoplast regeneration of the marine alga Bryopsis plumosa (Chlorophyta). Journal of Phycology 44(1): 103-112, 2008

Life history and cytology of the freshwater alga Chaetomorpha exilissima sp. nov (Cladophorales, Chlorophyta). Cytologia (Tokyo) 61(2): 179-188, 1996

Culture observation and molecular phylogenetic analysis on the blooming green alga Chaetomorpha valida Cladophorales, Chlorophyta from China. Chinese Journal of Oceanology and Limnology 31(3): 552-559, 2013

Cytotaxonomical studies of British marine species of Chaetomorpha. II. Chaetomorpha melagonium Kutz. Phykos (1 2) 17-22, 1972

Cyto taxonomical studies of british marine species of chaetomorpha part 2 chaetomorpha melagonium. Phykos 11(1-2): 17-22, 1972

From protoplasm to swarmer Protoplasts regeneration from disintegrated cells of the marine green alga Microdictyon umbilicatum. Phycologia 40(4 Supplement): 25, July, 2001

Life without a cell membrane: regeneration of protoplasts from disintegrated cells of the marine green alga Bryopsis plumosa. Journal of Cell Science 114(Pt 11): 2009-2014, 2001