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Degumming of ramie fibers by alkalophilic bacteria and their polysaccharide-degrading enzymes

Zheng, L.; Du, Y.; Zhang, J.

Bioresource Technology 78(1): 89-94

2001


ISSN/ISBN: 0960-8524
PMID: 11265793
DOI: 10.1016/s0960-8524(00)00154-1
Accession: 010422840

Three strains of alkalophilic bacteria, Bacillus sp. NT-39, NT-53 and NT-76, were selected for the degumming of ramie fibers and production of polysaccharide-degrading enzymes. After 48 h of incubation with the strains, the loss of the gum might amount to 5.0% or more of the fibers and a number of polysaccharide-degrading enzymes were secreted to the culture supernatants. The residual gum of the fibers decreased to 9.4% after 5 h of enzymatic degumming. Analysis of gum contents and enzyme activities revealed that pectate lyase and xylanase played an important role in the degradation of residual gum. Enzymatic degumming resulted in an increment of 5.4 ISO units in fiber brightness, whereas the reduction in bundle breaking tenacity of the fibers was less than 5.0%. The results confirmed that degumming of ramie fibers by alkalophilic bacteria and their enzymes had substantial advantages.

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