Section 11
Chapter 10,441

Determination of celecoxib in human plasma by normal-phase high-performance liquid chromatography with column switching and ultraviolet absorbance detection

Rose, M.J.; Woolf, E.J.; Matuszewski, B.K.

Journal of Chromatography. B Biomedical Sciences and Applications 738(2): 377-385


ISSN/ISBN: 1387-2273
PMID: 10718655
DOI: 10.1016/s0378-4347(99)00552-6
Accession: 010440460

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A method is described for the determination of celecoxib in human plasma. Samples were extracted using 3M Empore membrane extraction cartridges and separated under normal-phase HPLC conditions using a Nucleosil-NO2 (150X4.6 mm, 5 mum) column. Detection was accomplished using UV absorbance at 260 nm. The HPLC method included a column switching procedure, in which late eluting compounds were diverted to waste, to reduce run-time to 12 min. The assay was linear in the concentration range of 25-2000 ng/ml when 1-ml aliquots of plasma were extracted. Recoveries of celecoxib were greater than 91% over the calibration curve range. Intraday precision and accuracy for this assay were 5.7% C.V. or better and within 2.3% of nominal, respectively. The assay was used to analyze samples collected during human clinical studies.

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