+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Discriminating small molecule DNA binding modes by single molecule force spectroscopy



Discriminating small molecule DNA binding modes by single molecule force spectroscopy



Biophysical Journal 82(1 Part 2): 141a-142a



Drugs may interact with double stranded DNA via a variety of binding modes, each mode giving rise to a specific pharmacological function. Here we demonstrate the ability of single molecule force spectroscopy to discriminate between different interaction modes by measuring the mechanical properties of DNA and their modulation upon the binding of small molecules. Due to the unique topology of double stranded DNA and due to its base pair stacking pattern, DNA undergoes several well-characterised structural transitions upon stretching. We show that small molecule binding markedly affects these transitions in ways characteristic to the binding mode and that these effects can be detected at the level of an individual molecule. The minor groove binder berenil, the crosslinker cisplatin and the intercalator ethidium bromide are compared.

(PDF emailed within 0-6 h: $19.90)

Accession: 010477463

Download citation: RISBibTeXText

PMID: 11801245

DOI: 10.1016/s0014-5793(01)03257-4


Related references

Single-molecule force spectroscopy distinguishes target binding modes of calmodulin. Proceedings of the National Academy of Sciences of the United States of America 106(34): 14361-6, 2009

Investigation of the binding modes between AIE-active molecules and dsDNA by single molecule force spectroscopy. Nanoscale 7(19): 8939-8945, 2016

Single-Molecule Force Spectroscopy Reveals Multiple Binding Modes between DOPA and Different Rutile Surfaces. Chemphyschem 18(11): 1466-1469, 2016

Dynamics and multiple stable binding modes of DNA intercalators revealed by single-molecule force spectroscopy. Angewandte Chemie 51(8): 1811-1815, 2012

Mechanisms of small molecule-DNA interactions probed by single-molecule force spectroscopy. Nucleic Acids Research 44(9): 3971-3988, 2017

High density single-molecule-bead arrays for parallel single molecule force spectroscopy. Analytical Chemistry 84(11): 4907-4914, 2012

β-Connectin studies by small-angle x-ray scattering and single-molecule force spectroscopy by atomic force microscopy. Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics 83(5 Pt 1): 051919, 2011

Single-molecule force spectroscopy of the Aplysia cell adhesion molecule reveals two homophilic bonds. Biophysical Journal 103(4): 649-657, 2013

Discriminating Intercalative Effects of Threading Intercalator Nogalamycin, from Classical Intercalator Daunomycin, Using Single Molecule Atomic Force Spectroscopy. Plos One 11(5): E0154666, 2017

A functional single-molecule binding assay via force spectroscopy. Proceedings of the National Academy of Sciences of the United States of America 104(40): 15677-15681, 2007

Single molecule force spectroscopy of salt-dependent bacteriophage T7 gene 2.5 protein binding to single-stranded DNA. Journal of Biological Chemistry 281(50): 38689-38696, 2006

Binding and activity of topoisomerase 1B, studied using single-molecule force spectroscopy. Biophysical Journal 86(1): 324a, 2004

A force-spectroscopy-based single-molecule metal-binding assay. Chemphyschem 10(9-10): 1450-1454, 2009

Salt dependent binding of T4 gene 32 protein to single and double-stranded DNA: single molecule force spectroscopy measurements. Journal of Molecular Biology 349(2): 317-330, 2005

Single-Molecule Force Spectroscopy Reveals Self-Assembly Enhanced Surface Binding of Hydrophobins. Chemistry 24(37): 9224-9228, 2018