+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Docosahexaenoic acid is a potent inducer of apoptosis in HT-29 colon cancer cells



Docosahexaenoic acid is a potent inducer of apoptosis in HT-29 colon cancer cells



Prostaglandins Leukotrienes and Essential Fatty Acids 63(5): 301-308



Some studies have shown that dietary intake of polyunsaturated fatty acids of the n-3 series may have inhibitory effect on the growth of tumor cells both in vivo and in vitro. However, the cellular and molecular mechanisms by which n-3 fatty acids reduce the growth of tumor cells remain poorly understood. In the present studies, we compared the potency of a variety of n-3 and n-6 fatty acids in modulating the apoptotic cell death in HT-29 colon cancer cells. Of all fatty acids examined, we found that docosahexaenoic acid (22:6n-3; DHA) is a potent inducer of apoptosis in a time- and dose-dependent manner. Indomethacin, a cyclooxygenase inhibitor, is ineffective in blocking the apoptosis induced by DHA, suggesting that DHA-induced apoptosis in HT-29 cells is not mediated through the cyclooxygenase pathway. In contrast, the DHA-induced apoptosis is partially reversed by a synthetic antioxidant, butylated hydroxytoluene, indicating that lipid peroxidation may be involved in apoptotic signaling pathway induced by DHA. DHA treatment decreased bcl-2 levels in association with apoptosis, whereas bax levels remained unchanged. These results suggest that decreased expression of bcl-2 by DHA might increase the sensitivity of cells to lipid peroxidation and to programmed cell death.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 010490181

Download citation: RISBibTeXText

PMID: 11090257

DOI: 10.1054/plef.2000.0218


Related references

Conjugated docosahexaenoic acid inhibits lipid accumulation in rats. Journal of Nutritional Biochemistry 17(8): 518-524, 2006

6-18F-fluoro-L-dihydroxyphenylalanine positron emission tomography is superior to 123I-metaiodobenzyl-guanidine scintigraphy in the detection of extraadrenal and hereditary pheochromocytomas and paragangliomas: correlation with vesicular monoamine transporter expression. Journal of Clinical Endocrinology and Metabolism 95(6): 2800-2810, 2010

Boletus dermatitis: a new variant of flagellate erythema. Annals of Allergy Asthma and Immunology 115(3): 254-255, 2015

Synthesis of Titanium Dioxide by Microwave Plasma Torch. Journal of Nanoscience and Nanotechnology 15(4): 2829-2834, 2015

Thymoquinone arrests in vitro proliferation of canine osteosarcoma cells in the G1 phase. Proceedings of the American Association for Cancer Research Annual Meeting (41): 30, 2000

2,3',4,4',5'-Pentamethoxy-trans-stilbene, a resveratrol derivative, inhibits colitis-associated colorectal carcinogenesis in mice. British Journal of Pharmacology 160(6): 1352-1361, 2010

A Schiff base connectivity switch in sensory rhodopsin signaling. Proceedings of the National Academy of Sciences of the United States of America 105(42): 16159-16164, 2008

Docosahexaenoic acid sensitizes Ramos cells to Gamma-irradiation-induced apoptosis through involvement of PPAR-gamma activation and NF-kappaB suppression. Molecular and Cellular Biochemistry 317(1-2): 113-120, 2008

Docosahexaenoic acid provides protection from impairment of learning ability in Alzheimer's disease model rats. Journal of Neurochemistry 81(5): 1084-1091, 2002

Dietary fish oils modify the assembly of VLDL and expression of the LDL receptor in rabbit liver. Arteriosclerosis Thrombosis and Vascular Biology 18(9): 1490-1497, 1998

Acrolein-conjugated low-density lipoprotein induces macrophage foam cell formation. Atherosclerosis 227(1): 51-57, 2013

Hypotheticals, analogies, death's harms, and organ procurement. American Journal of Bioethics 9(8): 14-16, 2009

Brefeldin A interferes with peroxisomal protein sorting in the yeast Hansenula polymorpha. Febs Letters 411(1): 133-139, 1997

Colon cancer bears overexpression of OTUB1. Pathology Research and Practice 210(11): 770-773, 2014

6-halogenochromones bearing tryptamine: one-step access to potent and highly selective inhibitors of breast cancer resistance protein. Chemmedchem 7(7): 1177-1180, 2012