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Early selection for extreme resistance to potato virus Y and tobacco etch virus in potato using a beta-glucuronidase-tagged virus



Early selection for extreme resistance to potato virus Y and tobacco etch virus in potato using a beta-glucuronidase-tagged virus



Plant Breeding 119(4): 319-323, August



The Rysto gene from Solanum stoloniferum introduced into potato cultivars (Solanum tuberosum L. ssp. tuberosum) confers resistance to potato virus A, potato virus V and potato virus Y (PVY). In addition to PVY, tobacco etch virus (TEV) and a TEV construct that encodes beta-glucuronidase (TEV-GUS) were inoculated to determine the inheritance of resistance to these viruses in progenies obtained from potato cultivars containing the Rysto gene. While cultivars 'Karlena' and 'Delikat' were susceptible, 'Bettina' and clone 927eY were resistant to PVY, TEV and TEV-GUS, as determined by enzyme-linked immunosorbent assay, biotest and GUS assay, respectively. The segregation ratios obtained from the progenies of 'Bettina' X 'Delikat' and 927eY X 'Karlena' indicate that resistances to PVY and TEV are governed by one dominant gene or two genes tightly linked in coupling phase. Evidently, Rysto confers broad-spectrum resistance to potyviruses. TEV resistance could be reliably detected 4 days after inoculation with the TEV-GUS construct by GUS assay. Therefore, the GUS-tagged TEV construct can be used for early selection for resistances based on the gene Rysto or closely linked genes.

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Accession: 010507978

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