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Effect of acetate and pH on sunflower oil assimilation by Mucor circinelloides f. circinelloides



Effect of acetate and pH on sunflower oil assimilation by Mucor circinelloides f. circinelloides



Systematic and Applied Microbiology 22(1): 156-160



Mucor circinelloides f. circinelloides CBS 108.16 exhibited poor cell growth and substrate assimilation (32.5% of extracellular lipids) when grown with sunflower oil as sole carbon source. By contrast, in the presence of both sunflower oil (30 g/l) and sodium acetate 10 g/l), nearly complete utilization of both substrates (97.5% of sunflower oil, 100% of sodium acetate) occurred and biomass production was increased about five-fold to 23 g dry weight/l after 72 h. A significantly higher content of gamma-linolenic acid in the fungal neutral lipid fraction (5.2%) was also observed after 168 h of growth. These different patterns were attributed to the change in pH of the medium during cell growth in the presence and absence of acetate. In the absence of sodium acetate the pH decreased to 2.2, whereas in its presence it increased to about pH 8.0. During metabolism of sunflower oil in the presence of sodium acetate, the percentage of saturated fatty acids in the medium increased, suggesting a higher specificity of the fungal lipase for unsaturated fatty acids. For growth experiments in a small fermenter in the absence of sodium acetate, the gradual pH increase of sodium acetate containing medium was mimicked by an identical programme-controlled pH increase. Similar results as in the presence of sodium acetate were obtained. This observation indicated that the pH increase alone during cultivation was responsible for the increased sunflower oil utilization, biomass and GLA production.

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Accession: 010515570

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DOI: 10.1016/s0723-2020(99)80038-1


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