Effect of fibronectin on mouse embryo development and implantation in vitro
Cao, Y.J.; Zhang Chun, Y. Duan, E.K.; Zeng Guo Qing
Acta Zoologica Sinica 45(2): 187-193
Effects of fibronectin (FN) and its synthetic active peptide (Arg-Gly-Asp-Ser, RGDS) on mouse embryo development, blastocyst attachment and outgrowth on uterine epithelial cell monolayer or ectoplacental cone (EPC) on FN were studied by using in vitro culture models. The results showed that FN not only remarkably improved embryo development but also promoted attachment of blastocyst and EPC as well as outgrowth of primary and secondary trophoblast giant cells in vitro. On the other hand, these effects were inhibited by RGDS. These data suggest that FN plays a very important role in mouse embryo development and implantation and that this role is achieved by the recognition and binding between the FN active sites and FN receptors on the cell surface of embryo and ectoplacental cones.