+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Effect of liposome charge and PEG polymer layer thickness on cell-liposome electrostatic interactions

Effect of liposome charge and PEG polymer layer thickness on cell-liposome electrostatic interactions

Biochimica et Biophysica Acta 1564(2): 343-348, 31 August

Targeted drug delivery requires binding to (and subsequent uptake by) the carrier and target cell. In this paper, we calculate the work required to bring into contact liposomal carriers and cells as a function of the liposome and cell electrostatic characteristics. We find that cell-liposome adhesion is sensitive to the cell type and optimized at a cell to liposome charge ratio which depends on the degree of cell charge regulation. As a result, uptake (which is dependent on the occurrence of binding) is also optimized. Incorporation of a (poly)ethylene glycol (PEG) layer enhances liposome adhesion in cases where the cell-liposome interactions are repulsive, and suppresses adhesion in systems where the interactions are attractive. Our results, which are in agreement with experimental observations, show that electrostatic interactions may be designed to enable targeted drug delivery by liposomes to a specific cell population.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 010527903

Download citation: RISBibTeXText

PMID: 12175916

DOI: 10.1016/s0005-2736(02)00468-6

Related references

Liposome-cell interactions in vitro: effect of liposome surface charge on the binding and endocytosis of conventional and sterically stabilized liposomes. Biochemistry 37(37): 12875-12883, 1998

Effects of the protein corona on liposome-liposome and liposome-cell interactions. International Journal of Nanomedicine 11: 3049-3063, 2016

Polymer-liposome association induced by electrostatic interactions. European Biophysics Journal 32(3): 196, 2003

Effect of the electrostatic charge on the mechanism inducing liposome solubilization: a kinetic study by synchrotron radiation SAXS. Langmuir 20(8): 3074-3079, 2004

Liposome clearance in mice: The effect of a separate and combined presence of surface charge and polymer coating. International Journal of Pharmaceutics (Kidlington) 240(1-2): 95-102, 20 June, 2002

Poly(ethylene glycol) on the liposome surface: On the mechanism of polymer-coated liposome longevity. Biochimica et Biophysica Acta 1195(1): 11-20, 1994

The effects of liposome size and surface charge on liposome-mediated delivery of methotrexate-gamma-aspartate to cells in vitro. Biochimica et Biophysica Acta 820(1): 74-84, 1985

Kinetically controlled cellular interactions of polymer-polymer and polymer-liposome nanohybrid systems. Bioconjugate Chemistry 22(3): 466-474, 2011

Lipid-cell interactions. Liposome adsorption and cell-to-liposome lipid transfer are mediated by the same cell-surface sites. Biochimica et Biophysica Acta 804(1): 23-30, 1984

Liposome cell interactions in vitro and in vivo effects of surface charge cholesterol and gadolinium chloride. Studia Biophysica 128(3): 145-154, 1988

Role of the electrostatic depletion attraction on the structure of charged liposome-polymer mixtures. Physical Review. E Statistical Nonlinear and Soft Matter Physics 85(5 Pt 1): 051405, 2012

The preparation of sugar polymer-coated nanocapsules by the layer-by-layer deposition on the liposome. Langmuir 25(17): 10020-5, 2009