Home
  >  
Section 11
  >  
Chapter 10,531

Effect of nitric oxide synthase inhibition on high affinity Ca2+-ATPase during hypoxia in cerebral cortical neuronal nuclei of newborn piglets

Gavini, G.; Zanelli, S.A.; Ashraf, Q.M.; Mishra, O.P.; Delivoria Papadopoulos, M.

Brain Research 887(2): 385-390, 29 December

2000

ISSN/ISBN: 0006-8993
DOI: 10.1016/s0006-8993(00)03069-9
Accession: 010530064
Download citation:  
Text
  |  
BibTeX
  |  
RIS
Previous studies have shown that during hypoxia, neuronal nuclear high affinity Ca2+-ATPase activity is increased in the cerebral cortex of newborn piglets. The present study tests the hypothesis that pretreatment with N-nitro-L-arginine (NNLA) will prevent the hypoxia-induced increase in high affinity Ca2+-ATPase activity in cortical neuronal nuclear membrane of newborn piglets. We also tested the hypothesis that nitration is a mechanism of elevation of the high affinity Ca2+-ATPase activity during hypoxia. Studies were performed in five normoxic, five hypoxic, and six NNLA-pretreated (40 mg/kg) hypoxic newborn piglets. Cerebral cortical neuronal nuclei were isolated and the high affinity Ca2+-ATPase activity was determined. Further, normoxic samples were aliquoted into two sub-groups for in vitro nitration with 0.5 mM peroxynitrite and subsequent determination of the high affinity Ca2+-ATPase activity. The activity increased from 309+-40 nmol Pi/mg protein/h in the normoxic group to 520+-108 nmol Pi/mg protein/h in the hypoxic group (P<0.05). In the NNLA-pretreated group, the activity was 442+-53 nmol Pi/mg protein/h (P<0.05), which is 25% lower than in the hypoxic group. In the nitrated group the enzyme activity increased to 554+-59 nmol Pi/mg protein/h (P<0.05). Thus peroxynitrite-induced nitration in vitro increased the high affinity Ca2+-ATPase activity and NNLA administration in vivo partially prevented the hypoxia-induced increase in neuronal nuclear high affinity Ca2+-ATPase activity. We conclude that the hypoxia-induced increase in nuclear membrane high affinity Ca2+-ATPase activity is NO-mediated and that nitration of the enzyme is a mechanism of its modification.

PDF emailed within 0-6 h: $19.90




Related References

Gavini, G.; Zanelli, S.A.; Ashraf, Q.M.; Mishra, O.P.; Delivoria-Papadopoulos, M. 2000: Effect of nitric oxide synthase inhibition on high affinity Ca(2+)-ATPase during hypoxia in cerebral cortical neuronal nuclei of newborn piglets Brain Research 887(2): 385-390
Gavini, G.; Qayyum, I.; Mcgowan, J.E.; Mishra, O.P.; Delivoria Papadopoulos, M. 2000: Effect of inhibition of nitric oxide synthase on high-affinity Ca++-ATPase activity in cerebral cortical nuclei of newborn piglets during hypoxia Pediatric Research 47(4 Part 2): 458A
Ballesteros, J.R.; Zubrow, A.B.; Zhu, A.; Delivoria Papadopoulos, M.; Mishra, O.P. 2000: Effect of nitric oxide synthase inhibition on Ca++-influx in cerebral cortical neuronal nuclei of newborn piglets Pediatric Research 47(4 Part 2): 455A
Parikh, N.A.; Fritz, K.I.; Zhu, A.; Mishra, O.P.; Delivoria Papadopoulos, M. 2002: Effect of neuronal nitric oxide synthase inhibition on DNA fragmentation in neuronal nuclei during hypoxia in newborn piglets Journal of Investigative Medicine 50(2): 183A
Sorrentino, D.F.; Zubrow, A.B.; Haider, S.H.; Delivoria Papadopoulos, M.; Mishra, O.P. 2003: Effect of neuronal nitric oxide synthase inhibition on CaM kinase IV activity during hypoxia in neuronal nuclei of newborn piglets Pediatric Research 53(4 Part 2): 50A
Mishra, O.P.; Delivoria-Papadopoulos, M. 2001: Effect of graded hypoxia on high-affinity Ca2+-ATPase activity in cortical neuronal nuclei of newborn piglets Neurochemical Research 26(12): 1335-1341
Zubrow, A.B.; Fritz, K.I.; Phokela, S.S.; Mishra, O.P.; Delivoria Papadopoulos, M. 2000: Effect of nitric oxide synthase inhibition on the expression of Bax and Bcl-2 proteins in neuronal nuclei during hypoxia in newborn piglets Pediatric Research 47(4 Part 2): 467A
Amjad, M.; Qayyum, I.; Mcgowan, J.E.; Mishra, O.P.; Delivoria Papadopoulos, M. 1999: Effect of graded hypoxia on high-affinity Ca++-ATPase activity in cerebral cortical nuclei of newborn piglets Pediatric Research 45(4 Part 2): 182A
Zubrow, A.B.; Delivoria-Papadopoulos, M.; Fritz, K.I.; Mishra, O.P. 2004: Effect of neuronal nitric oxide synthase inhibition on CA2+/calmodulin kinase kinase and CA2+/calmodulin kinase IV activity during hypoxia in cortical nuclei of newborn piglets Neuroscience 125(4): 937-945
Mishra, O.P.; Delivoria-Papadopoulos, M. 2006: Effect of neuronal nitric oxide synthase inhibition on caspase-9 activity during hypoxia in the cerebral cortex of newborn piglets Neuroscience Letters 401(1-2): 81-85
Parikh, N.A.; Katsetos, C.D.; Legido, A.; Fritz, K.I.; Mishra, O.P. 2003: Effect of neuronal nitric oxide synthase inhibition on Caspase-3 expression during hypoxia in the cerebral cortex of newborn piglets Pediatric Research 53(4 Part 2): 63A
Zubrow, A.B.; Ashraf, Q.M.; Zhu, A.; Delivoria Papadopoulos, M.; Mishra, O.P. 2001: Effect of nitric oxide synthase inhibition on nuclear Ca++/calmodulin-dependent protein kinase IV activity during hypoxia in neuronal nuclei of newborn piglets Pediatric Research 49(4 Part 2): 444A
Groenendaal, F.; Mishra, O.P.; McGowan, J.E.; Hoffman, D.J.; Delivoria-Papadopoulos, M. 1997: Function of cell membranes in cerebral cortical tissue of newborn piglets after hypoxia and inhibition of nitric oxide synthase Pediatric Research 42(2): 174-179
Groenendaal, F.; Mishra, O.P.; Mcgowan, J.E.; Hoffman, D.J.; Delivoria Papadopoulos, M. 1994: Effect of cerebral nitric oxide synthase inhibition on brain cell membrane Na+, K+-ATPase activity during hypoxia in newborn piglets Pediatric Research 37(4 Part 2): 207A
Mishra, O.P.; Mishra, R.; Ashraf, Q.M.; Delivoria-Papadopoulos, M. 2006: Nitric oxide-mediated mechanism of neuronal nitric oxide synthase and inducible nitric oxide synthase expression during hypoxia in the cerebral cortex of newborn piglets Neuroscience 140(3): 857-863
Zubrow, A.B.; Fritz, K.I.; Zhu, A.; Mishra, O.P.; Delivoria Papadopoulos, M. 2003: Effect of neuronal nitric oxide synthase inhibition on nuclear Ca++/calmodulin-dependent protein kinase kinase activity during hypoxia in neuronal nuclei of newborn piglets Pediatric Research 53(4 Part 2): 562A
Sorrentino, D.F.; Fritz, K.I.; Zhu, A.; Delivoria Papadopoulos, M.; Mishra, O.P. 2003: The effect of neuronal nitric oxide synthase inhibition on NMDA receptor glycine-binding site characteristics during hypoxia in the cerebral cortex of newborn piglets Pediatric Research 53(4 Part 2): 562A
Mishra, O.P.; Zubrow, A.B.; Anday, E.K.; Kubin, J.; Delivoria Papadopoulos, M. 2003: Effect of neuronal nitric oxide synthase inhibition on MAP kinase-1 activity in neuronal nuclei of newborn piglets Pediatric Research 53(4 Part 2): 546A
Mishra, O.P.; Ashraf, Q.M.; Delivoria-Papadopoulos, M. 2009: Tyrosine phosphorylation of neuronal nitric oxide synthase (nNOS) during hypoxia in the cerebral cortex of newborn piglets: the role of nitric oxide Neuroscience Letters 462(1): 64-67
Zanelli, S.A.; Akhter, W.; Ashraf, Q.M.; Mishra, O.P.; Delivoria Papadopoulos, M. 2000: Effect of nitric oxide synthase inhibition on DNA fragmentation in neuronal nuclei of newborn piglets Pediatric Research 47(4 Part 2): 475A