+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn

+ Translate
+ Recently Requested

Effects of activin A and TGF-beta 1 on differentiation of osteoblast-like MC 3 T 3-E 1 cells

Effects of activin A and TGF-beta 1 on differentiation of osteoblast-like MC 3 T 3-E 1 cells

Shikwa Gakuho 101(6): 521-532

To elucidate the role activin A and transforming growth factor (TGF)-beta 1, which play important roles as coupling factors in bone remodeling, we investigated the effects of these cytokines on the differentiation of osteoblast-like MC 3 T 3-E 1 cells by analyzing the formation of bone nodules, alkaline phosphatase (ALP) activity and osteopontin (OPN) mRNA expression. Activin A increased the mineralized bone nodule accompanied by an acceleratedincrease in ALP activity, and it slightly stimulated OPN mRNA expression at the late stage of culture (21apprx28 days). TGF-beta 1 inhibited an increase in ALP activity and the formation of bone nodules in both untreated and activuin A treated cells. The inhibitory effect was predominant at the early stage of culture (7apprx14 days) with activin A. TGF-beta 1 increased OPN mRNA expression, which was synergistically cnhanced by the simultaneous treatment with activin A. When the cells were pretreated with TGF-beta 1, the synergistic effect by activin A on OPN mRNA expression was observed at the late stage of culture. While, when the cells were pretreated with activin A, the synergistic effect by TGF-beta 1 was observed at the early stage of culture. These results suggest that the cytokines regulate the differentiation of osteoblast-like cells depending on the stage of differentiation.

(PDF emailed within 1 workday: $29.90)

Accession: 010544087

Download citation: RISBibTeXText

Related references

Inhibitory effects of activin-A on osteoblast differentiation during cultures of fetal rat calvarial cells. Journal of Cellular Biochemistry 75(2): 206-214, Nov 1, 1999

The effects of TGF-beta on the proliferation and differentiation of human osteoblast-like cells. Hua Xi Kou Qiang Yi Xue Za Zhi 16(1): 65-8, 72, 2002

Effects of transforming growth factor-beta and activin A on vitamin D3-induced monocytic differentiation of myeloid leukemia cells. Anticancer Research 11(1): 181-186, 1991

Porcine fetal enamel matrix derivative stimulates proliferation but not differentiation of pre-osteoblastic 2T9 cells, inhibits proliferation and stimulates differentiation of osteoblast-like MG63 cells, and increases proliferation and differentiation of normal human osteoblast NHOst cells. Journal of Periodontology 71(8): 1287-1296, 2000

Overexpression of activin beta(C) or activin beta(E) in the mouse liver inhibits regenerative deoxyribonucleic acid synthesis of hepatic cells. Endocrinology 144(8): 3497-3504, 2003

The thyroid hormone receptor (TR) beta-selective agonist GC-1 inhibits proliferation but induces differentiation and TR beta mRNA expression in mouse and rat osteoblast-like cells. Calcified Tissue International 84(4): 324-333, 2009

Dual Effects of Liquiritigenin on the Proliferation of Bone Cells: Promotion of Osteoblast Differentiation and Inhibition of Osteoclast Differentiation. PhytoTherapy Research 29(11): 1714-1721, 2016

Osteoblast migration into type I collagen gel and differentiation to osteocyte-like cells within a self-produced mineralized matrix: a novel system for analyzing differentiation from osteoblast to osteocyte. Bone 52(1): 102-110, 2013

Smad signaling pathways are used by TGF-Beta and activin to regulate differentiation of corneal/conjunctival epithelial cells. IOVS 40(4): S759, March 15, 1999

The role of activin A, betacellulin and hepatocyte growth factor in beta cell differentiation Lessons from AR42J cells. Experimental & Clinical Endocrinology & Diabetes 105(4): A12, 1997

IGF-I and TGF-beta 1 incorporated in a poly(D,L-lactide) implant coating stimulates osteoblast differentiation and collagen-1 production but reduces osteoblast proliferation in cell culture. Journal of Biomedical Materials Research. Part B, Applied Biomaterials 65(1): 157-162, 2003

Inhibition by vitamin D3 of erythroid differentiation of human leukemia line cells induced by transforming growth factor beta or erythroid differentiation factor (activin A). Leukemia Research 15(8): 721-726, 1991