+ Site Statistics
References:
52,654,530
Abstracts:
29,560,856
PMIDs:
28,072,755
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Effects of adenoviral up-regulation of bcl-2 on oxidative stress and graft coronary artery disease in rat heart transplants



Effects of adenoviral up-regulation of bcl-2 on oxidative stress and graft coronary artery disease in rat heart transplants



Transplantation 76(2): 382-386



Bcl-2 has been shown to have antioxidant properties. Early oxidative stress is an important antigen-independent factor that contributes to the development of graft coronary artery disease (GCAD). We hypothesized that adenoviral up-regulation of bcl-2 would decrease early oxidative stress and inhibit GCAD after heart transplantation. PVG rat hearts were treated with adenovirus carrying the human bcl-2 gene (AdvBcl-2) or null adenovirus (AdvNull) then transplanted into the abdomens of PVG recipients. After 4 days of reperfusion to allow adenoviral gene expression, grafts were retransplanted into ACI rat recipients and reperfused for 4 or 8 hours or 90 days (cyclosporine A 7.5 mg/kg on postoperative day [POD] 0-9). Production of tumor necrosis factor (TNF)-alpha after 4 hours and oxidized glutathione (GSSG) after 8 hours indicated development of oxidative stress. 90-day allografts were assessed for GCAD by way of computerized morphometry. Over-expression of bcl-2 at the time of allograft reperfusion was confirmed by Western blotting. Whereas AdvNull-treated hearts demonstrated elevated TNF-alpha levels after 4 hours and increased GSSG after 8 hours of reperfusion, AdvBcl-2-treated hearts were no different from nontransplanted hearts. AdvBcl-2 treatment also resulted in decreased luminal narrowing and intima-to-media ratio at POD 90. Bcl-2 over-expression interrupts the development of oxidative stress in reperfused rat-heart allografts. Early up-regulation of bcl-2 also decreases GCAD, indicating the importance of early oxidative stress and the role that bcl-2 may play in the long-term function of heart transplants.

(PDF emailed within 0-6 h: $19.90)

Accession: 010544362

Download citation: RISBibTeXText

PMID: 12883197

DOI: 10.1097/01.TP.0000072367.22036.2F


Related references

Adenoviral upregulation of Bcl-2 decreases oxidative stress and graft coronary artery disease in rat heart transplants. Journal of Heart & Lung Transplantation 21(1): 71-72, January, 2002

Differences in oxidative stress markers based on the aetiology of heart failure: comparison of oxidative stress in patients with and without coronary artery disease. Free Radical Research 43(12): 1159-1166, 2010

Oxidative stress and mitochondrial damage in coronary artery bypass graft surgery: effects of antioxidant treatments. Comprehensive Therapy 27(2): 108-116, 2001

Combined polymorphisms in oxidative stress genes predict coronary artery disease and oxidative stress in coronary angiography patients. Annals of Human Genetics 76(6): 435-447, 2013

Oxidative injury and antioxidants in coronary artery bypass graft surgery: off-pump CABG significantly reduces oxidative stress. Clinica Chimica Acta; International Journal of Clinical Chemistry 375(1-2): 147-152, 2006

Effects of ozone therapy on haemostatic and oxidative stress index in coronary artery disease. European Journal of Pharmacology 691(1-3): 156-162, 2012

Estimated contribution of coronary artery bypass graft surgery to the decline in coronary heart disease mortality: The Minnesota heart survey. Journal of the American College of Cardiology 24(1): 95-103, 1994

Comparison of effects of simvastatin versus atorvastatin on oxidative stress in patients with coronary heart disease. Clinical Cardiology 33(4): 222-227, 2010

Effects of black tea consumption on plasma catechins and markers of oxidative stress and inflammation in patients with coronary artery disease. Free Radical Biology and Medicine 38(4): 499-506, 2005

Correlations between oxidative DNA damage, oxidative stress and coenzyme Q10 in patients with coronary artery disease. International Journal of Medical Sciences 9(8): 621-626, 2013

The effects of stress on heart rate variability in patients with coronary artery disease. Medicine & Science in Sports & Exercise 29(5 SUPPL ): S179, 1997

Effects of clopidogrel therapy on oxidative stress, inflammation, vascular function, and progenitor cells in stable coronary artery disease. Journal of Cardiovascular Pharmacology 63(4): 369-374, 2014

Effects of long-term nicorandil administration on endothelial function, inflammation, and oxidative stress in patients without coronary artery disease. Journal of Cardiovascular Pharmacology 51(3): 311-316, 2008

Coronary bypass graft surgery in the beating heart abolishes inflammation and oxidative stress. Journal of Molecular & Cellular Cardiology 31(6): A121, June, 1999