+ Site Statistics
References:
52,654,530
Abstracts:
29,560,856
PMIDs:
28,072,755
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Effects of aging and sex steroids on the localization of T cell subsets in the ovary of chicken, Gallus domesticus



Effects of aging and sex steroids on the localization of T cell subsets in the ovary of chicken, Gallus domesticus



General and Comparative Endocrinology 114(1): 28-35



The goal of this study was to determine the effects of aging and sex steroids on the frequency of T cells in hen ovary. Cryostat sections of ovarian tissues of immature and laying hens and those of immature hens treated with or without diethylstilbestrol (DES) or progesterone were immunostained for T cells using mouse anti-chicken CD3 (antigen of mature T cells), CD4 (antigen of helper T cells), and CD8 (antigen of cytotoxic T cells) monoclonal antibodies. Positive cells were observed under a light microscope and counted using a computer-assisted image analyzer. The frequency of CD3(+), CD4(+), and CD8(+) cells in the ovarian stroma and theca of primary follicles was significantly greater in young laying hens than in immature and old laying hens (P < 0.01). The CD4:CD8 ratio was significantly higher in the ovarian stroma of old laying hens than that of immature hens (P < 0.01), which was due to a greater decrease of CD8(+) cells than of CD4(+) cells. The frequency of CD3(+), CD4(+), and CD8(+) cells was significantly greater in the stroma and theca of primary follicles of DES-treated birds than in those of progesterone-treated and control birds (P < 0.01). Progesterone had no significant effect on the population of each subset of T cells. These results suggest that T cell frequency increases in association with sexual maturation and decreases thereafter during aging with an increase of CD4:CD8 ratio. Also, it is likely that estrogen is one of the factors which stimulates the influx of T cells in the hen ovary.

(PDF emailed within 0-6 h: $19.90)

Accession: 010544676

Download citation: RISBibTeXText

PMID: 10094856

DOI: 10.1006/gcen.1998.7232


Related references

Effects of age and gonadal steroids on the localization of T cell subsets in the epididymis of male chickens, Gallus domesticus. Journal of Poultry Science 42(3): 193-204, 2005

The effects of age and sex steroids on the macrophage population in the ovary of the chicken, Gallus domesticus. Journal of Reproduction and Fertility 114(2): 253-258, 1999

Effects of age and gonadal steroids on the localization of antigen presenting cells in the epididymis of the male chicken, Gallus domesticus. Journal of Reproduction and Development 52(3): 363-371, 2006

Production of chicken progeny (Gallus gallus domesticus) from interspecies germline chimeric duck (Anas domesticus) by primordial germ cell transfer. Biology of Reproduction 86(4): 101-101, 2012

Genetic characterization of Bhutanese native chickens based on an analysis of Red Junglefowl (Gallus gallus gallus and Gallus gallus spadecieus), domestic Southeast Asian and commercial chicken lines (Gallus gallus domesticus). Genetics and Molecular Biology 35(3): 603-609, 2012

Cell number and sex ratio in unfertilized chicken eggs (Gallus gallus domesticus). Journal of Experimental Zoology. Part A, Ecological Genetics and Physiology 309(1): 47-54, 2007

Action of growth factors on the ovary in the domestic chicken (Gallus domesticus). Contraception, Fertilite, Sexualite 22(9): 577-586, 1994

Organization of facial nerve parasympathetic cell columns in the chicken gallus gallus var domesticus. Medical Science Research 16(10): 499-500, 1988

Bioavailability and biochemical effects of diclofenac sodium 0.1% ophthalmic solution in the domestic chicken (Gallus gallus domesticus). Veterinary Ophthalmology (): -, 2016

Toxicity effects of toad (Rhinella jimi Stevaux, 2002) venom in chicken (Gallus gallus domesticus). Thescientificworldjournal 2014: 851473-851473, 2015

Influence of aging and estrogen treatment on the IgY concentration in the egg yolk of chicken, Gallus domesticus. Japanese Poultry Science 37(5): 280-288, 2000

Biosynthetic pathways of testosterone and estradiol-17 beta in slices of the embryonic ovary and testis of the chicken (Gallus domesticus). General and Comparative Endocrinology 73(1): 69-79, 1989

Immunocytochemical localization of L-dopa and dopamine in the brain of the chicken (Gallus domesticus). Journal of Comparative Neurology 346(1): 97-118, 1994

Localization of the telomeric (TTAGGG)n sequence in chicken (Gallus domesticus) chromosomes. Cytogenetics and Cell Genetics 65(3): 190-193, 1994