+ Site Statistics
References:
52,654,530
Abstracts:
29,560,856
PMIDs:
28,072,755
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Effects of aging on central and peripheral mammalian clocks



Effects of aging on central and peripheral mammalian clocks



Proceedings of the National Academy of Sciences of the United States of America 99(16): 10801-6



Circadian organization changes with age, but we do not know the extent to which age-related changes are the result of alterations in the central pacemakers, the peripheral oscillators, or the coupling mechanisms that hold the system together. By using transgenic rats with a luciferase (luc) reporter, we assessed the effects of aging on the rhythm of expression of the Period 1 (Per1) gene in the suprachiasmatic nucleus (SCN) and in peripheral tissues. Young (2 months) and aged (24-26 months) Per1-luc transgenic rats, entrained to light-dark cycles, were killed, and tissues were removed and cultured. Per1-luc expression was measured from 10 tissues. In the SCN, the central mammalian pacemaker, Per1-luc expression was robustly rhythmic for more than 7 weeks in culture. The only difference between SCN rhythmicity in young and old rats was a small but significant age-related shortening of the free-running period. Circadian rhythmicity in some peripheral tissues was unaffected by aging, whereas rhythmicity in other tissues was either phase advanced relative to the light cycle or absent. Those tissues that were arrhythmic could be induced to oscillate by application of forskolin, suggesting that they retained the capacity to oscillate but were not being appropriately driven in vivo. Overall, the results provide new insights into the effects of aging on the mammalian circadian system. Aging seems to affect rhythms in some but not in all tissues and may act primarily on interactions among circadian oscillators, perhaps attenuating the ability of the SCN to drive damped oscillators in the periphery.

(PDF emailed within 0-6 h: $19.90)

Accession: 010544689

Download citation: RISBibTeXText

PMID: 12149444

DOI: 10.1073/pnas.152318499


Related references

The mammalian circadian timing system: organization and coordination of central and peripheral clocks. Annual Review of Physiology 72: 517-549, 2010

Daily NO rhythms in peripheral clocks in aging male Wistar rats: protective effects of exogenous melatonin. Biogerontology 17(5-6): 859-871, 2016

Effects of chronic jet lag on the central and peripheral circadian clocks in CBA/N mice. Chronobiology International 31(2): 189-198, 2015

Circadian lessons from peripheral clocks: is the time of the mammalian pacemaker up?. Proceedings of the National Academy of Sciences of the United States of America 101(16): 5699-5700, 2004

The mammalian circadian timing system: synchronization of peripheral clocks. Cold Spring Harbor Symposia on Quantitative Biology 76: 39-47, 2012

Mammalian cultured cells as a model system of peripheral circadian clocks. Journal of Biochemistry 134(6): 785-790, 2004

Rhythms of mammalian body temperature can sustain peripheral circadian clocks. Current Biology 12(18): 1574-1583, September 17, 2002

Integrative coordination of circadian mammalian diversity: neuronal networks and peripheral clocks. Progress in Neurobiology 54(1): 87-97, 1998

Central and peripheral circadian clocks in mammals. Annual Review of Neuroscience 35: 445-462, 2012

Crosstalk between central and peripheral biological clocks. Nihon Rinsho. Japanese Journal of Clinical Medicine 70(7): 1127-1132, 2012

Hypothalamic integration of central and peripheral clocks. Nature Reviews. Neuroscience 2(7): 521-526, 2001

Central and peripheral clocks in cardiovascular and metabolic function. Annals of Medicine 38(8): 552-559, 2006

The regulation of central and peripheral circadian clocks in humans. Obesity Reviews 10 Suppl 2: 25-36, 2010

Central and peripheral clocks, light, day length, and reproduction. Biology of Reproduction 66(Supplement 1): 78-79, 2002

Interaction of central and peripheral clocks in physiological regulation. Progress in Brain Research 199: 163-181, 2013