+ Site Statistics
References:
52,654,530
Abstracts:
29,560,856
PMIDs:
28,072,755
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Effects of agmatine on polyamine metabolism and the growth of prostate tumor cells



Effects of agmatine on polyamine metabolism and the growth of prostate tumor cells



Journal of Biochemistry & Molecular Biology 32(2): 173-180, March 31



The effects of agmatine on the enzymes responsible for the biosynthesis of polyamines, the resultant levels of polyamines, and their effect on the growth of DU145 human prostate tumor cells were investigated. When agmatine was added to the medium, ornithine decarboxylase (ODC, EC 4.1.1.17) activity was substantially reduced, but S-adenosylmethionine decarboxylase (SAMDC, EC 4.1.1.50) activity increased markedly. These changes in ODC and SAMDC activities were the result of an induction of ODC-antizyme and a decreased turnover rate of SAMDC in the presence of agmatine. Accordingly, there was a decrease in the intracellular levels of putrescine and spermidine but an increase in the intracellular level of spermine. Cell growth was markedly inhibited by agmatine treatment and this inhibition was not recovered by the addition of putrescine or spermidine. Our results suggest that agmatine alters the intracellular amounts of polyamine in the cells, closely related to the inhibition of cell growth.

(PDF emailed within 1 workday: $29.90)

Accession: 010544746

Download citation: RISBibTeXText


Related references

Inhibitory effect of agmatine on proliferation of tumor cells by modulation of polyamine metabolism. Acta Pharmacologica Sinica 26(5): 616-622, 2005

Effects of exogenous agmatine in human leukemia HMC-1 and HL-60 cells on proliferation, polyamine metabolism and cell cycle. Leukemia Research 35(9): 1248-1253, 2011

Effects of agmatine accumulation in human colon carcinoma cells on polyamine metabolism, DNA synthesis and the cell cycle. Biochimica et Biophysica Acta 1745(1): 111-123, 2005

Agmatine inhibits the proliferation of rat hepatoma cells by modulation of polyamine metabolism. Journal of Hepatology 39(5): 793-799, 2003

Effects of polyamine-enriched diets on growth, development and polyamine metabolism of rats. European Journal of Clinical Investigation 29(SUPPL 1): 21, April, 1999

Effects of a polyamine-enriched diet on growth, development and polyamine metabolism in rats. Gastroenterology 120(5 Supplement 1): A 302, April, 2001

The effects of nerve growth factor on polyamine metabolism in PC12 cells. Journal of Neurochemistry 40(5): 1271-1277, 1983

Effects of Ri TL-DNA from Agrobacterium rhizogenes and the inhibitors of polyamine synthesis on growth, floral development, sexual organogenesis and polyamine metabolism in tobacco. Plant Science Limerick 80(1-2): 131-144, 1991

Effects of L-valine on growth and polyamine metabolism in human colon carcinoma cells. Biochimica Et Biophysica Acta. 1379(1): 151-160,. 8, 1998

Effects of cyclosporin a on growth and polyamine metabolism of molt 4 t lymphoblastic leukemia cells. British Journal of Cancer 64(2): 255-258, 1991

Agmatine suppresses mesangial cell proliferation by modulating polyamine metabolism. Tohoku Journal of Experimental Medicine 210(2): 145-151, 2006

Effects of garlic thioallyl derivatives on growth, glutathione concentration, and polyamine formation of human prostate carcinoma cells in culture. American Journal of Clinical Nutrition 66(2): 398-405, 1997

Effects of changes in polyamine metabolism on DNA methylation during growth inhibition of human colonic cancer cells. Proceedings of the Nutrition Society 58(3): 72A, 1999