+ Site Statistics
References:
52,654,530
Abstracts:
29,560,856
PMIDs:
28,072,755
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Effects of all-trans retinoic acid and interferon alpha in peripheral neuroectodermal tumor cell cultures and xenografts



Effects of all-trans retinoic acid and interferon alpha in peripheral neuroectodermal tumor cell cultures and xenografts



International Journal of Oncology 13(5): 943-949



Peripheral neuroectodermal tumors (PNET) have an unsatisfactory outcome when treated with standard approaches. Among novel treatments, the use of biological response modifiers has rarely been reported in this group of malignancies. We have previously demonstrated that both alltrans retinoic acid (ATRA) and interferon alpha (IFNalpha) can inhibit proliferation of human PNET cells and that ATRA can up-regulate IFNalpha receptor expression in vitro. In this study we evaluated the anti-tumor effects of ATRA and IFNalpha in PNET cells in vitro and in a human PNET xenograft model, using CHP100 cells. A synergistic inhibitory effect of ATRA and IFNalpha was observed on CHP100 cells in vitro. On the contrary, a significant inhibition of tumor growth was observed in mice treated with ATRA alone, whereas neither IFNalpha nor the combination of ATRA and IFNalpha, reached a statistically significant anti-tumor effect. Histologic examination of tumors revealed the presence of necrosis upon treatment with IFNalpha, whereas almost no necrosis, but a more differentiated morphology, confirmed by electron microscopy analysis, was associated with the ATRA containing treatments. Taken together these data show an in vitro and in vivo antitumor activity of ATRA in human PNET cells, although no synergism of ATRA and IFNalpha was observed in our xenograft model.

(PDF emailed within 0-6 h: $19.90)

Accession: 010544923

Download citation: RISBibTeXText

PMID: 9772283

DOI: 10.3892/ijo.13.5.943


Related references

All-trans retinoic acid and interferon-alpha for treatment of human renal cell carcinoma multicellular tumor spheroids. Urologia Internationalis 73(1): 47-53, 2004

Antiproliferative effects of alpha and beta interferon in combination with 5 fluorouracil cisplatin and cis and trans retinoic acid in two non small cell lung carcinoma cell lines. Proceedings of the American Association for Cancer Research Annual Meeting 32: 228, 1991

Antiproliferative effects of interferon alpha-2a in combination with all trans retinoic acid or cisplatin in human ovarian carcinoma cell lines. Proceedings of the American Association for Cancer Research Annual Meeting 37(0): 293, 1996

All-trans retinoic acid potentiates the inhibitory effects of interferon-alpha on parental and P210-transfected murine myeloid cell line. Experimental Hematology (Charlottesville) 26(8): 713, 1998

13-cis-retinoic acid , all-trans-retinoic acid or alpha-interferon partially inhibit the proliferation of small cell lung carcinoma and colonic carcinoma in vitro. Clinical & Investigative Medicine 16(4 SUPPL ): B92, 1993

Efficacity and pharmacokinetic of all trans retinoic acid (ATRA)/interferon alpha-2a (INF alpha-2a) in metastatic renal cell cancer. Journal de Pharmacie Clinique 18(4): 318-320, 1999

Activation of retinoic acid receptor alpha mediates the effects of all-trans- and 9-cis-retinoic acid on the cholinergic properties of a murine septal cell line. Society for Neuroscience Abstracts 20(1-2): 80, 1994

Binding of 9-cis-retinoic acid and all-trans-retinoic acid to retinoic acid receptors alpha, beta, and gamma. Retinoic acid receptor gamma binds all-trans-retinoic acid preferentially over 9-cis-retinoic acid. Journal of Biological Chemistry 269(24): 16689-16695, 1994

All-trans- and 9-cis-retinoic acid enhance the cholinergic properties of a murine septal cell line: evidence that the effects are mediated by activation of retinoic acid receptor-alpha. Journal of Neurochemistry 65(1): 50-58, 1995

Suppression of in vitro maintenance of non-promyelocytic myeloid leukemia clonogenic cells by all-trans retinoic acid: modulating effects of dihydroxylated vitamin D3, alpha interferon and 'stem cell factor'. Leukemia Research 21(1): 51-58, 1997

All-trans-retinoic acid suppresses interferon-gamma and tumor necrosis factor-alpha; a possible therapeutic agent for rheumatoid arthritis. Rheumatology International 26(9): 810-817, 2005

All-trans-retinoic acid suppresses interferon-gamma and tumor necrosis factor-alpha; a possible therapeutic agent for rheumatoid arthritis. Rheumatology International 26(9): 810-817, 2006

Effects of dexamethasone, all-trans retinoic acid, vitamin D(3) and interferon-alpha on FO myeloma cells. ChemoTherapy 50(4): 190-193, 2004

13-cis-retinoic acid or all-trans-retinoic acid plus interferon-alpha in recurrent cervical cancer: a Southwest Oncology Group phase II randomized trial. Gynecologic Oncology 71(3): 386-390, 1999