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Effects of an inhibitor of tripeptidyl peptidase II (Ala-Ala-Phe-chloromethylketone) and its combination with an inhibitor of the chymotrypsin-like activity of the proteasome (PSI) on apoptosis, cell cycle and proteasome activity in U937 cells

Bury, M.; Młynarczuk, I.; Pleban, E.; Hoser, G.; Kawiak, J.; Wójcik, C.

Folia Histochemica et Cytobiologica 39(2): 131-132

2001


ISSN/ISBN: 0239-8508
PMID: 11374791
Accession: 010545397

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AAF-AMC is not a specific TPP II substrate, since it is also hydrolyzed by purified proteasomes. Moreover, AAF-cmk, claimed to be a specific TPP II inhibitor, also inhibits the chymotrypsin-like activity of the proteasome. While AAF-cmk itself is mildly cytostatic to U-937 cells and induces cell cycle block in G1, its combination with PSI does not induce an increase in the cytostatic/cytotoxic effects.

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