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Effects of tumor necrosis factor-alpha on the synthesis of DNA, the secretion of matrix metalloproteinases/tissue inhibitors of metalloproteinases, and the activity of invasive migration in cultured vascular smooth muscle cells



Effects of tumor necrosis factor-alpha on the synthesis of DNA, the secretion of matrix metalloproteinases/tissue inhibitors of metalloproteinases, and the activity of invasive migration in cultured vascular smooth muscle cells



Journal of Health Science 48(4): 354-358



To address the question of whether or not tumor necrosis factor-alpha (TNF-alpha) regulates the functions of vascular smooth muscle cells (SMCs), dense or sparse cultures of the cells derived from human aorta were treated with TNF-alpha or TNF-alpha neutralizing antibody (TNF-alpha Ab). The incorporation of (3H)thymidine into the acid-insoluble fraction of SMCs was significantly inhibited by TNF-alpha, but stimulated by TNF-alpha Ab only when the cells had a high cell density. TNF-alpha significantly increased the accumulation of matrix metalloproteinase-1 and -3 (MMT-1 and -3, respectively) in the conditioned medium of dense SMCs, but does not affect that of tissue inhibitors of metalloproteinases-1 (TIMP-1); MMP-9 and TIMP-2 were undetectable. The invasive migration of SMCs determined by a Transwell system was stimulated by neither TNF-alpha nor TNF-alpha Ab. Taking these results together, it is suggested that TNF-alpha regulates DNA and MMP synthesis in dense SMCs but does not affect their invasive migration.

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