+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Electrogenic reduction of the primary electron donor P700 by plastocyanin in photosystem I complexes

Electrogenic reduction of the primary electron donor P700 by plastocyanin in photosystem I complexes

FEBS Letters 500(3): 172-176, 6 July

An electrometric technique was used to investigate electron transfer between spinach plastocyanin (Pc) and photooxidized primary electron donor P700 in photosystem I (PS I) complexes from the cyanobacterium Synechocystis sp. PCC 6803. In the presence of Pc, the fast unresolvable kinetic phase of membrane potential generation related to electron transfer between P700 and the terminal iron-sulfur acceptor F(B) was followed by additional electrogenic phases in the microsecond and millisecond time scales, which contribute approximately 20% to the overall electrogenicity. These phases are attributed to the vectorial electron transfer from Pc to the protein-embedded chlorophyll dimer P700(+) within the PsaA/PsaB heterodimer. The observed rate constant of the millisecond kinetic phase exhibited a saturation profile at increasing Pc concentration, suggesting the formation of a transient complex between Pc and PS I with the dissociation constant K(d) of about 80 microM. A small but detectable fast electrogenic phase was observed at high Pc concentration. The rate constant of this phase was independent of Pc concentration, indicating that it is related to a first-order process.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 010575598

Download citation: RISBibTeXText

PMID: 11445080

DOI: 10.1016/s0014-5793(01)02615-1

Related references

Electrogenic reduction of the primary electron donor P700+ in photosystem I by redox dyes. Febs Letters. 414(2): 196, 1997

FTIR study of the primary electron donor of photosystem I (P700) revealing delocalization of the charge in P700(+) and localization of the triplet character in (3)P700. Biochemistry 38(36): 11585-11592, 1999

Ftir Study of the Primary Electron Donor of Photosystem I (P700) Revealing Delocalization of the Charge in P700 + and Localization of the Triplet Character in 3 P700. Biochemistry 38(36): 11585-11592, 1999

The two histidine axial ligands of the primary electron donor chlorophylls (P700) in photosystem I are similarly perturbed upon P700+ formation. Biochemistry 41(37): 11200-11210, 2002

P700: The primary electron donor of photosystem I. Biochimica et Biophysica Acta 1507(1-3): 61-79, 30 October, 2001

Stark effect in P700, the primary electron donor of Photosystem I. Febs Letters 286(1-2): 110-112, 1991

Electron transfer between plastocyanin and p700 in various types of photosystem i complex. Plant & Cell Physiology 30(1): 85-90, 1989

Redox potentials and spectroscopic properties of the primary electron donor p700 of photosystem I. 2007

Reduction of the primary donor P700 of photosystem I during steady-state photosynthesis under low light in Arabidopsis. Photosynthesis Research 99(1): 37-47, 2008

Primary donor photo-oxidation in photosystem I: a re-evaluation of (P700(+) - P700) Fourier transform infrared difference spectra. Biochemistry 40(43): 12943-9, 2001

Electron transfer from plastocyanin to P700. Function of a subunit of photosystem I reaction center. FEBS letters 111(1): 79-82, 1980

Excitation and Electron Transfer from Selectively Excited Primary Donor Chlorophyll (P700) in a Photosystem I Reaction Center. Journal of Physical Chemistry A 101(4): 597-604, 1997

Identification of kinetic parameters of plastocyanin and p700 interaction in chloroplasts and pigment protein complexes of photosystem 1. Photosynthetica 24(3): 495-501, 1990

Characterization of the primary electron donor of photosystem I, P700, by electrochemistry and Fourier transform infrared (FTIR) difference spectroscopy. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 52(1): 107-121, 1996

Species dependence of the redox potential of the primary electron donor p700 in photosystem I of oxygenic photosynthetic organisms revealed by spectroelectrochemistry. Plant & Cell Physiology 52(5): 815-823, 2011