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Establishment of a fluorescent polymerase chain reaction method for the detection of the SARS-associated coronavirus and its clinical application



Establishment of a fluorescent polymerase chain reaction method for the detection of the SARS-associated coronavirus and its clinical application



Chinese Medical Journal 116(7): 988-990



Objective: To establish a fluorescent polymerase chain reaction (F-PCR) method for detecting the coronavirus related to severe acute respiratory syndrome (SARS) and to evaluate its value for clinical application. Methods: The primers and the fluorescence-labeled probe were designed and synthesized according to the published sequence of the SARS-associated coronavirus genes. A F-PCR diagnosis kit for detecting the coronavirus was developed, and 115 clinical nasopharyngeal gargling liquid samples were tested. Results: The sequence of PCR amplified products completely matched the related sequence of the SARS-associated coronavirus genome. Forty-nine out of 67 samples from identified SARS patients and 8 of 18 samples from persons having close contact with SARS patients showed positive results. All 30 samples from healthy controls were negative. Conclusion: The F-PCR method established may be a rapid, accurate and efficient way for screening and for the early diagnosis of SARS patients.

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Accession: 010603891

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PMID: 12890368


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