+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Estradiol-17beta is produced in bovine corpus luteum



Estradiol-17beta is produced in bovine corpus luteum



Biology of Reproduction 65(6): 1634-1639



The aim of this study was to investigate the expression of cytochrome P450 aromatase (aromatase) mRNA, its activity, and estradiol-17beta (estradiol) secretion in bovine corpus luteum (CL) during the estrous cycle. Expression of aromatase mRNA was examined in CL at the early, mid, late, and regressed luteal stages by using a reverse transcription-polymerase chain reaction. Aromatase mRNA was detected in all luteal stages examined, although aromatase expression was significantly lower during the early and regressed luteal phases compared to the mid and late luteal phases. Moreover, cultured midluteal cells clearly converted exogenous [(3)H]androstenedione into estradiol, and an aromatase inhibitor significantly inhibited this conversion. To characterize the local release of estradiol within the CL during the estrous cycle, an in vitro microdialysis system (MDS) of CL was conducted. Estradiol in MDS perfusate was confirmed by a reverse-phase high-performance liquid chromatography in combination with enzyme immunoassays. Basal release of estradiol from microdialyzed CL did not change during the estrous cycle. Additionally, when freshly prepared midluteal cells were exposed to estradiol (10(-14) to 10(-9) M), estradiol stimulated prostaglandin (PG) F(2alpha) secretion (P < 0.05), although it did not affect progesterone and oxytocin secretion. The overall results indicate that estradiol is produced locally in bovine CL throughout the estrous cycle, and they suggest that estradiol plays a role in regulating PGF(2alpha) production in CL as an autocrine/paracrine factor.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 010607368

Download citation: RISBibTeXText

PMID: 11717122


Related references

Estradiol-17 Is Produced in Bovine Corpus Luteum. Biology of Reproduction 65(6): 1634-1639, 2001

Progesterone and androgen secretion by isolated cultured bovine corpus luteum cells: effect of LH, hCG, PRL, estradiol 17beta and testosterone. Folia Histochemica et Cytobiologica 23(1-2): 11-16, 1985

The Effect of 17beta-Estradiol on Corpus Luteum Function in Sheep. Biology of Reproduction 2(1): 48-52, 1970

Radioimmunoassay of progesterone, 17-hydroxyprogesterone, estradiol-17beta and prostaglandin F in human corpus luteum. Steroids 26(3): 299-310, 1975

Modulation of the uptake and retention of estradiol-17beta in the ovine corpus luteum by luteinizing hormone. Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine 149(2): 537-540, 1975

Synthesis of 17beta-estradiol by isolated ovarian tissues of the pregnant rat: aromatization in the corpus luteum. Endocrinology 99(2): 573-579, 1976

Cellular and biochemical changes in the rabbit corpus luteum after withdrawal of 17beta-estradiol. I. Paradoxical increase in RNA synthesis. Biology of Reproduction 17(1): 16-22, 1977

A Mechanism for Regression of the Rabbit Corpus Luteum: Uterine-Induced Loss of Luteal Responsiveness to 17beta-Estradiol. Biology of Reproduction 15(4): 511-518, 1976

17beta-Estradiol receptor and progesterone and 20alpha-hydroxy-4-pregnen-3-one content of the developing corpus luteum of the rabbit. Endocrinology 101(6): 1744-1750, 1977

Cellular and biochemical changes in the rabbit corpus luteum after withdrawal of 17beta-estradiol. II. Radioautographic analysis of [3H] uridine incorporation. Biology of Reproduction 17(1): 23-33, 1977

The effect of estradiol on bovine corpus luteum function. Biology of Reproduction 36(Suppl. 1): 185, 1987

The effect of human chorionic gonadotropin and estradiol-17beta on the maintenance of the human corpus luteum of early pregnancy. American Journal of Obstetrics and Gynecology 128(5): 469-475, 1977

Mechanisms for rescue of corpus luteum during pregnancy: gene expression in bovine corpus luteum following intrauterine pulses of prostaglandins E1 and F2α. Biology of Reproduction 98(4): 465-479, 2018

Differences of microvascular endothelium in the bovine corpus luteum of pregnancy and the corpus luteum of the estrous cycle. Biology of the Cell 87(3): 179-188, 1996

Survival role of locally produced acetylcholine in the bovine corpus luteum. Biology of Reproduction 80(4): 823-832, 2009