+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Evaluation of phagocytosis and arachidonate metabolism by alveolar macrophages and recruited neutrophils from F344xBN rats of different ages



Evaluation of phagocytosis and arachidonate metabolism by alveolar macrophages and recruited neutrophils from F344xBN rats of different ages



Mechanisms of Ageing & Development 122(15): 1899-1913



The incidence of infectious respiratory diseases increases with aging. Resident alveolar macrophages (AMs) and recruited leukocytes (PMNL) mediate cellular defense against bacterial infections in the lung, and phagocytosis and lipid mediator synthesis are important components of their antimicrobial capacity. The objective of this study was to determine if either phagocytic capacity or lipid mediator generation declines with normal aging, in either AMs or PMNL recruited to a site of inflammation. The F344xBN rat hybrid has a lower incidence of pathologies associated with aging, particularly up to 20 months; animals aged 6, 12 and 18 months were chosen to evaluate changes associated with normal aging. As previously reported for peripheral blood leukocytes, phagocytosis by recruited PMNL declined with aging: recruited PMNL from 18 months rats showed a significantly decreased capacity to phagocytose live Klebsiella pneumoniae bacteria, compared to PMNL from 6 months rats. Surprisingly, however, the phagocytic capacity of AMs increased with aging: the phagocytic index of AMs from 18 months rats was more than three times that of AMs from 6 months rats. The capacity of AMs and recruited PMNL to release arachidonic acid or synthesize leukotrienes or prostaglandins did not change with aging. This study demonstrates that, although phagocytosis by recruited PMNL declines with aging, other aspects of immune function do not decline, and may actually increase, with normal aging. These results suggest that impaired phagocytosis by recruited PMNL may be an important component of the increased susceptibility to infectious respiratory diseases during normal aging.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 010617429

Download citation: RISBibTeXText

PMID: 11557288

DOI: 10.1016/s0047-6374(01)00322-0


Related references

Protein tyrosine kinase activity and the influence of gender in phagocytosis and tumor necrosis factor secretion in alveolar macrophages and lung-recruited neutrophils. Shock 6(6): 426-433, 1996

Relation of respiratory burst and arachidonate metabolism during phagocytosis by guinea pig alveolar macrophages. Journal of Laboratory and Clinical Medicine 99(6): 908-916, 1982

The flow cytometric evaluation of phagocytosis by equine peripheral blood neutrophils and pulmonary alveolar macrophages. Veterinary Journal 156(2): 107-116, Sept, 1998

Tyrosine Phosphorylation Regulates Phagocytosis By Alveolar Macrophages And Pmns Recruited To The Lung. Shock 5: 51-52, 1996

The arachidonate cascade and alveolar macrophages but not neutrophils mediate bronchoconstriction by fmlp in guinea pigs. British Journal of Pharmacology 96(SUPPL): 78P, 1989

Phagocytosis by human alveolar macrophages and neutrophils. Chest 76(3): 371-372, 1979

Phagocytosis of Candida albicans by rabbit alveolar macrophages and guinea pig neutrophils. Sabouraudia 15(2): 171-177, 1977

Sexual differences in the kinetics of endotoxin-stimulated increases of inducible nitric oxide synthase mRNA in both alveolar macrophages and recruited neutrophils, in vivo. Journal of Investigative Medicine 44(1): 73A, 1996

Poractant alfa (Curosurf®) increases phagocytosis of apoptotic neutrophils by alveolar macrophages in vivo. Respiratory Research 13: 17, 2012

Comparison of arachidonate metabolism by alveolar macrophages from bighorn and domestic sheep. Inflammation 15(1): 43-54, 1991

Comparative phagocytosis of Cryptococcus neoformans a and alpha strains by human neutrophils, monocytes, and rat alveolar macrophages. Abstracts of the General Meeting of the American Society for Microbiology 96(0): 91, 1996

Differential dependence of arachidonate metabolism on protein kinase c in alveolar and peritoneal macrophages. Clinical Research 38(2): 440A, 1990

Differences in protein kinase c activated arachidonate metabolism between peritoneal and alveolar macrophages. Clinical Research 37(4): 920A, 1989

Different patterns of arachidonate metabolism in autologous human blood monocytes and alveolar macrophages. Journal of Immunology 142(2): 602-608, 1989

Multiple defects in arachidonate metabolism in alveolar macrophages from young asymptomatic smokers. Journal of Laboratory and Clinical Medicine 114(6): 662-673, 1989